Display of Native Antigen on cDC1 That Have Spatial Access to Both T and B Cells Underlies Efficient Humoral Vaccination.
Animals
Antigen Presentation
Autoantigens
/ immunology
B-Lymphocytes
/ immunology
Cell Differentiation
Cells, Cultured
Cytokines
/ metabolism
Dendritic Cells
/ immunology
Immunity, Humoral
Lectins, C-Type
/ genetics
Mice
Mice, Inbred C57BL
Mice, Knockout
Receptors, Immunologic
/ genetics
Th1 Cells
/ immunology
Th2 Cells
/ immunology
Vaccination
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
01 10 2020
01 10 2020
Historique:
received:
14
05
2020
accepted:
24
07
2020
pubmed:
26
8
2020
medline:
30
3
2021
entrez:
26
8
2020
Statut:
ppublish
Résumé
Follicular dendritic cells and macrophages have been strongly implicated in presentation of native Ag to B cells. This property has also occasionally been attributed to conventional dendritic cells (cDC) but is generally masked by their essential role in T cell priming. cDC can be divided into two main subsets, cDC1 and cDC2, with recent evidence suggesting that cDC2 are primarily responsible for initiating B cell and T follicular helper responses. This conclusion is, however, at odds with evidence that targeting Ag to Clec9A (DNGR1), expressed by cDC1, induces strong humoral responses. In this study, we reveal that murine cDC1 interact extensively with B cells at the border of B cell follicles and, when Ag is targeted to Clec9A, can display native Ag for B cell activation. This leads to efficient induction of humoral immunity. Our findings indicate that surface display of native Ag on cDC with access to both T and B cells is key to efficient humoral vaccination.
Identifiants
pubmed: 32839238
pii: jimmunol.2000549
doi: 10.4049/jimmunol.2000549
pmc: PMC7504891
doi:
Substances chimiques
Autoantigens
0
Clec9a protein, mouse
0
Cytokines
0
Lectins, C-Type
0
Receptors, Immunologic
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1842-1856Informations de copyright
Copyright © 2020 by The American Association of Immunologists, Inc.
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