Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche.
Adaptation, Physiological
/ physiology
Adherens Junctions
/ physiology
Animals
Basement Membrane
/ physiology
Cell Adhesion
Cell Movement
/ physiology
Cell-Matrix Junctions
/ physiology
Collagen
/ metabolism
Male
Muscle Fibers, Skeletal
/ physiology
Muscle, Skeletal
/ physiology
Physical Conditioning, Animal
/ physiology
Rats
Rats, Wistar
Resistance Training
/ methods
Sarcoplasmic Reticulum
/ physiology
Sedentary Behavior
Telocytes
/ physiology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 08 2020
24 08 2020
Historique:
received:
24
01
2020
accepted:
04
08
2020
entrez:
26
8
2020
pubmed:
26
8
2020
medline:
8
1
2021
Statut:
epublish
Résumé
The present study shows chronic adjustments in the myotendinous junction (MTJ) in response to different ladder-based resistance training (LRT) protocols. Thirty adult male Wistar rats were divided into groups: sedentary (S), calisthenics (LRT without additional load [C]), and resistance-trained (LRT with extra weight [R]). We demonstrated longer lengths of sarcoplasmatic invaginations in the trained groups; however, evaginations were seen mainly in group R. We showed a greater thickness of sarcoplasmatic invaginations in groups C and R, in addition to greater evaginations in R. We also observed thinner basal lamina in trained groups. The support collagen layer (SCL) adjacent to the MTJ and the diameters of the transverse fibrils were larger in R. We also discovered a niche of telocytes in the MTJ with electron micrographs of the plantar muscle and with immunostaining with CD34+ in the gastrocnemius muscle near the blood vessels and pericytes. We concluded that the continuous adjustments in the MTJ ultrastructure were the result of tissue plasticity induced by LRT, which is causally related to muscle hypertrophy and, consequently, to the remodeling of the contact interface. Also, we reveal the existence of a collagen layer adjacent to MTJ and discover a new micro anatomic location of telocytes.
Identifiants
pubmed: 32839490
doi: 10.1038/s41598-020-70971-6
pii: 10.1038/s41598-020-70971-6
pmc: PMC7445244
doi:
Substances chimiques
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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