Utility of p16 and HPV testing in oropharyngeal squamous cell carcinoma: An institutional review.
Aged
Aged, 80 and over
Alphapapillomavirus
/ genetics
Biomarkers, Tumor
/ genetics
Carcinoma, Squamous Cell
/ genetics
Cyclin-Dependent Kinase Inhibitor p16
/ metabolism
Female
Human Papillomavirus DNA Tests
/ methods
Humans
Lymph Nodes
/ virology
Male
Middle Aged
Oropharyngeal Neoplasms
/ genetics
Papillomavirus Infections
/ genetics
RNA, Viral
/ genetics
HPV
cytology
head and neck
p16
squamous cell carcinoma
Journal
Diagnostic cytopathology
ISSN: 1097-0339
Titre abrégé: Diagn Cytopathol
Pays: United States
ID NLM: 8506895
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
20
07
2020
accepted:
07
08
2020
pubmed:
28
8
2020
medline:
1
10
2021
entrez:
27
8
2020
Statut:
ppublish
Résumé
In the US, 60% to 80% of oropharyngeal squamous cell carcinomas (OPSCCs) are associated with human papillomavirus (HPV). However, until recently, no consensus existed about when and how to test for HPV in patients with head and neck cancers. We aimed to evaluate the use of p16 and HPV testing at our institution because p16 immunohistochemistry is reportedly a reliable surrogate marker for HPV detection in OPSCCs. We identified all cases at our institution of primary or metastatic squamous cell carcinoma (SCC) of the head and neck with a concurrent p16 immunostain analysis from January 1, 2013, through August 31, 2018. Patient demographic data, tumor characteristics, p16 result, and any HPV result (in situ hybridization and E6 and E7 RNA test) were captured. We identified 104 patients. Most primary tumors (53/57 [93.0%]) and metastases (40/47 [85.1%]) were positive for p16. Thirty-seven cases (35.6%) had reflex high-risk HPV (HR HPV) testing performed. Of the 35 p16-positive cases, 6 had discrepant HR HPV results (p16 p16 immunohistochemistry accurately identifies HPV-positive OPSCC. Cytology specimens have an important role in characterizing SCC of unknown origin. HR HPV testing is not routinely required, and results may be discrepant with p16 findings.
Substances chimiques
Biomarkers, Tumor
0
Cyclin-Dependent Kinase Inhibitor p16
0
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
54-59Informations de copyright
© 2020 Wiley Periodicals LLC.
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