Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning.

Animals Astrocytes / metabolism Fear / psychology Humans Mice Neuronal Plasticity / physiology
astrocyte local translation neuroglial interactions perisynaptic astrocyte processes

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
25 08 2020
Historique:
received: 05 03 2020
revised: 08 07 2020
accepted: 05 08 2020
entrez: 27 8 2020
pubmed: 28 8 2020
medline: 3 6 2021
Statut: ppublish

Résumé

Local translation is a conserved mechanism conferring cells the ability to quickly respond to local stimuli. In the brain, it has been recently reported in astrocytes, whose fine processes contact blood vessels and synapses. Yet the specificity and regulation of astrocyte local translation remain unknown. We study hippocampal perisynaptic astrocytic processes (PAPs) and show that they contain the machinery for translation. Using a refined immunoprecipitation technique, we characterize the entire pool of ribosome-bound mRNAs in PAPs and compare it with the one expressed in the whole astrocyte. We find that a specific pool of mRNAs is highly polarized at the synaptic interface. These transcripts encode an unexpected molecular repertoire, composed of proteins involved in iron homeostasis, translation, cell cycle, and cytoskeleton. Remarkably, we observe alterations in global RNA distribution and ribosome-bound status of some PAP-enriched transcripts after fear conditioning, indicating the role of astrocytic local translation in memory and learning.

Identifiants

DOI: 10.1016/j.celrep.2020.108076 PMID: 32846133 PMC: PMC7450274
pubmed: 32846133
pii: S2211-1247(20)31061-5
doi: 10.1016/j.celrep.2020.108076
pmc: PMC7450274
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

108076

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests The authors declare no conflict of interest.

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Auteurs

Noémie Mazaré (N)

Physiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France; Doctoral School No. 158, Pierre and Marie Curie University, 75005 Paris, France.

Marc Oudart (M)

Physiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France; Doctoral School No. 158, Pierre and Marie Curie University, 75005 Paris, France.

Julien Moulard (J)

Doctoral School No. 158, Pierre and Marie Curie University, 75005 Paris, France; Neuroglial Interactions in Cerebral Physiopathology Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France.

Giselle Cheung (G)

Neuroglial Interactions in Cerebral Physiopathology Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France.

Romain Tortuyaux (R)

Physiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France.

Philippe Mailly (P)

Orion Imaging Facility, Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France.

David Mazaud (D)

Neuroglial Interactions in Cerebral Physiopathology Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France.

Alexis-Pierre Bemelmans (AP)

CEA, DRF, Institut de Biologie François Jacob, Molecular Imaging Research Center (MIRCen), 92265 Fontenay-aux-Roses, France; CNRS, CEA, Université Paris-Sud, Université Paris-Saclay, Neurodegenerative Diseases Laboratory (UMR9199), 92265 Fontenay-aux-Roses, France.

Anne-Cécile Boulay (AC)

Physiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France.

Corinne Blugeon (C)

Genomic Facility, Institut de Biologie de l'ENS (IBENS), Département de Biologie, École Normale Supérieure, CNRS, INSERM, Université PSL, 75005 Paris, France.

Laurent Jourdren (L)

Genomic Facility, Institut de Biologie de l'ENS (IBENS), Département de Biologie, École Normale Supérieure, CNRS, INSERM, Université PSL, 75005 Paris, France.

Stéphane Le Crom (S)

Genomic Facility, Institut de Biologie de l'ENS (IBENS), Département de Biologie, École Normale Supérieure, CNRS, INSERM, Université PSL, 75005 Paris, France; Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratory of Computational and Quantitative Biology (LCQB), 75005 Paris, France.

Nathalie Rouach (N)

Neuroglial Interactions in Cerebral Physiopathology Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France.

Martine Cohen-Salmon (M)

Physiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France. Electronic address: martine.cohen-salmon@college-de-france.fr.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Local Translation in Perisynaptic Astrocytic...
questionsmedicales.fr › Cellules › Névroglie › Astrocytes › Local Translation in Perisynaptic Astrocytic...
questionsmedicales.fr › Comportement et mécanismes comportementaux › Émotions › Peur › Local Translation in Perisynaptic Astrocytic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Local Translation in Perisynaptic Astrocytic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Local Translation in Perisynaptic Astrocytic...
questionsmedicales.fr › Phénomènes physiologiques de l'appareil locomoteur et du système nerveux › Phénomènes physiologiques du système nerveux › Plasticité neuronale › Local Translation in Perisynaptic Astrocytic...