Antimicrobial Effectiveness of Calcium Silicate Sealers against a Nutrient-Stressed Multispecies Biofilm.

antimicrobial properties bioceramic sealers biofilms calcium silicate sealers confocal laser scanning microscopy dental materials endodontic sealers

Journal

Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588

Informations de publication

Date de publication:
24 Aug 2020
Historique:
received: 29 07 2020
revised: 16 08 2020
accepted: 21 08 2020
entrez: 28 8 2020
pubmed: 28 8 2020
medline: 28 8 2020
Statut: epublish

Résumé

This study compared the antimicrobial efficacy of calcium silicate sealers (BioRoot RCS and Total Fill BC) and conventional sealers (AH Plus and Tubli-seal) against planktonic bacteria and a nutrient-stressed multispecies biofilm. Antimicrobial properties of freshly mixed sealers were investigated using the direct contact test (DCT) and a nutrient-stressed multispecies biofilm comprised of five endodontic strains. Antimicrobial activity was determined using quantitative viable counts and confocal laser scanning microscopy (CLSM) analysis with live/dead staining. The pH of the sealers was analysed over a period of 28 days in Hanks Balanced Salt Solution (HBSS). Analysis of variance (ANOVA) with Tukey tests and the Kruskal-Wallis test were used for data analysis with a significance of 5%. All endodontic sealers exhibited significant antimicrobial activity against planktonic bacteria ( Calcium silicate sealers exhibited effective antimicrobial properties. This was demonstrated by superior biofilm inhibition capacity and microbial killing, with strong alkalizing activity compared to epoxy-based and zinc oxide-eugenol-based sealers.

Identifiants

pubmed: 32846942
pii: jcm9092722
doi: 10.3390/jcm9092722
pmc: PMC7563851
pii:
doi:

Types de publication

Journal Article

Langues

eng

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Auteurs

Rahul Bose (R)

Centre for Host Microbiome Interactions, King's College London Dental Institute, Floor 17, Tower Wing, Guy's Dental Hospital, London Bridge, London SE1 9RT, UK.

Konstantinos Ioannidis (K)

Centre for Host Microbiome Interactions, King's College London Dental Institute, Floor 17, Tower Wing, Guy's Dental Hospital, London Bridge, London SE1 9RT, UK.

Federico Foschi (F)

Centre for Host Microbiome Interactions, King's College London Dental Institute, Floor 17, Tower Wing, Guy's Dental Hospital, London Bridge, London SE1 9RT, UK.
Department of Therapeutic Dentistry I. M., Sechenov First Moscow State Medical University, 119146 Moscow, Russia.

Abdulaziz Bakhsh (A)

Centre for Host Microbiome Interactions, King's College London Dental Institute, Floor 17, Tower Wing, Guy's Dental Hospital, London Bridge, London SE1 9RT, UK.
Department of Restorative Dentistry, Faculty of Dentistry, Umm Al-Qura University, Makkah 24381, Saudi Arabia.

Robert D Kelly (RD)

Centre for Host Microbiome Interactions, King's College London Dental Institute, Floor 17, Tower Wing, Guy's Dental Hospital, London Bridge, London SE1 9RT, UK.

Sanjukta Deb (S)

Centre for Host Microbiome Interactions, King's College London Dental Institute, Floor 17, Tower Wing, Guy's Dental Hospital, London Bridge, London SE1 9RT, UK.

Francesco Mannocci (F)

Centre for Host Microbiome Interactions, King's College London Dental Institute, Floor 17, Tower Wing, Guy's Dental Hospital, London Bridge, London SE1 9RT, UK.

Sadia Ambreen Niazi (SA)

Centre for Host Microbiome Interactions, King's College London Dental Institute, Floor 17, Tower Wing, Guy's Dental Hospital, London Bridge, London SE1 9RT, UK.

Classifications MeSH