Measuring the Inducible, Replication-Competent HIV Reservoir Using an Ultra-Sensitive p24 Readout, the Digital ELISA Viral Outgrowth Assay.
DEVO
HIV
IUPM
QVOA
outgrowth
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2020
2020
Historique:
received:
28
02
2020
accepted:
21
07
2020
entrez:
28
8
2020
pubmed:
28
8
2020
medline:
1
5
2021
Statut:
epublish
Résumé
Quantifying the inducible HIV reservoir provides an estimate of the frequency of quiescent HIV-infected cells in humans as well as in animal models, and can help ascertain the efficacy of latency reversing agents (LRAs). The quantitative viral outgrowth assay (QVOA) is used to measure inducible, replication competent HIV and generate estimations of reservoir size. However, traditional QVOA is time and labor intensive and requires large amounts of lymphocytes. Given the importance of reproducible and accurate assessment of both reservoir size and LRA activity in cure strategies, efforts to streamline the QVOA are of high priority. We developed a modified QVOA, the Digital ELISA Viral Outgrowth or DEVO assay, with ultra-sensitive p24 readout, capable of femtogram detection of HIV p24 protein in contrast to the picogram limitations of traditional ELISA. For each DEVO assay, 8-12 × 10
Identifiants
pubmed: 32849659
doi: 10.3389/fimmu.2020.01971
pmc: PMC7423995
doi:
Substances chimiques
HIV Core Protein p24
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1971Subventions
Organisme : NIAID NIH HHS
ID : R01 AI134363
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI126619
Pays : United States
Informations de copyright
Copyright © 2020 Stuelke, James, Kirchherr, Allard, Baker, Kuruc, Gay, Margolis and Archin.
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