Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular Disease.

cell line diagnosis foot-and-mouth disease virus vesicular viruses virus isolation

Journal

Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658

Informations de publication

Date de publication:
2020
Historique:
received: 14 04 2020
accepted: 12 06 2020
entrez: 28 8 2020
pubmed: 28 8 2020
medline: 28 8 2020
Statut: epublish

Résumé

The most sensitive cell culture system for the isolation of foot-and-mouth disease virus (FMDV) is primary bovine thyroid (BTY) cells. However, BTY cells are seldom used because of the challenges associated with sourcing thyroids from FMDV-negative calves (particularly in FMD endemic countries), and the costs and time required to regularly prepare batches of cells. Two continuous cell lines, a fetal goat tongue cell line (ZZ-R 127) and a fetal porcine kidney cell line (LFBK-α

Identifiants

pubmed: 32851014
doi: 10.3389/fvets.2020.00426
pmc: PMC7401924
doi:

Types de publication

Journal Article

Langues

eng

Pagination

426

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007036
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007037
Pays : United Kingdom

Informations de copyright

Copyright © 2020 Gray, Wood, Henry, Azhar, King and Mioulet.

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Auteurs

Ashley R Gray (AR)

Vesicular Disease Reference Laboratory, The Pirbright Institute, Surrey, United Kingdom.

Britta A Wood (BA)

Vesicular Disease Reference Laboratory, The Pirbright Institute, Surrey, United Kingdom.

Elisabeth Henry (E)

Vesicular Disease Reference Laboratory, The Pirbright Institute, Surrey, United Kingdom.

Mehreen Azhar (M)

Vesicular Disease Reference Laboratory, The Pirbright Institute, Surrey, United Kingdom.

Donald P King (DP)

Vesicular Disease Reference Laboratory, The Pirbright Institute, Surrey, United Kingdom.

Valérie Mioulet (V)

Vesicular Disease Reference Laboratory, The Pirbright Institute, Surrey, United Kingdom.

Classifications MeSH