Tissue Plasminogen Activator Loaded PCL Nanofibrous Scaffold Promoted Nerve Regeneration After Sciatic Nerve Transection in Male Rats.
Axonal regeneration
In vivo evaluations
Peripheral nerve
Plasma irradiation
Tissue plasminogen activator
Journal
Neurotoxicity research
ISSN: 1476-3524
Titre abrégé: Neurotox Res
Pays: United States
ID NLM: 100929017
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
28
05
2020
accepted:
20
08
2020
revised:
19
08
2020
pubmed:
28
8
2020
medline:
15
12
2021
entrez:
28
8
2020
Statut:
ppublish
Résumé
According to the studies, damages to the peripheral nerve as a result of a trauma or acute compression, stretching, or burns accounts for a vast range of discomforts which strongly impressed the patient's life quality. Applying highly potent biomolecules and growth factors in the damaged nerve site would promote the probability of nerve regeneration and functional recovery. Tissue plasminogen activator (tPA) is one of the components that can contribute importantly to degenerating and regenerating the peripheral nerves following the injuries occurred and the absence of this biomolecule hinders the recoveries of the nerves. This technique would guarantee the direct accessibility of tPA for the regenerating axons. Structural, physical, and in vitro cytotoxicity evaluations were done before in vivo experiments. In this study, twenty-four mature male rats have been exploited. The rats have been classified into four groups: controls, axotomy, axotomy + scaffold, and axotomy + tPA-loaded scaffold. Four, 8, and 12 weeks post-surgical, the sciatic functional index (SFI) has been measured. After 12 weeks, the spinal cord, sciatic nerve, and dorsal root ganglion specimens have been removed and stereological procedures, immunohistochemistry, and gene expression have been used to analyze them. Stereological parameters, immunohistochemistry of GFAP, and gene expression of S100, NGF, and BDNF were significantly enhanced in tPA-loaded scaffold group compared with axotomy group. The most similarity was observed between the results of control group and tPA-loaded scaffold group. According to the results, a good regeneration of the functional nerve tissues in a short time was observed as a result of introducing tPA.
Identifiants
pubmed: 32852719
doi: 10.1007/s12640-020-00276-z
pii: 10.1007/s12640-020-00276-z
doi:
Substances chimiques
Tissue Plasminogen Activator
EC 3.4.21.68
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
413-428Subventions
Organisme : Shahid Beheshti University of Medical Sciences
ID : 91-6124
Références
Akassoglou K, Kombrinck KW, Degen JL, Strickland S (2000) Tissue plasminogen activator–mediated fibrinolysis protects against axonal degeneration and demyelination after sciatic nerve injury. J Cell Biol 149(5):1157–1166
pubmed: 10831618
pmcid: 2174825
Alvarez-Perez MA, Guarino V, Cirillo V, Ambrosio L (2010) Influence of gelatin cues in PCL electrospun membranes on nerve outgrowth. Biomacromolecules 11(9):2238–2246
pubmed: 20690634
Böttcher H, Eisbrenner K, Fritz S, Kindermann G, Kraxner F, McCallum I, Obersteiner M (2009) An assessment of monitoring requirements and costs of ‘Reduced Emissions from Deforestation and Degradation’. Carbon Balance and Management 4(1):7
pubmed: 19709413
pmcid: 2741441
Cao H, Liu T, Chew SY (2009) The application of nanofibrous scaffolds in neural tissue engineering. Adv Drug Deliv Rev 61(12):1055–1064
pubmed: 19643156
Chew SY, Mi R, Hoke A, Leong KW (2008) The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation. Biomaterials 29(6):653–661
pubmed: 17983651
Chong EJ, Phan TT, Lim IJ, Zhang Y, Bay BH, Ramakrishna S, Lim CT (2007) Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomater 3(3):321–330
pubmed: 17321811
Frattini F, Pereira Lopes FR, Almeida FM, Rodrigues RF, Boldrini LC, Tomaz MA, Baptista AF, Melo PA, Martinez AMB (2012) Mesenchymal stem cells in a polycaprolactone conduit promote sciatic nerve regeneration and sensory neuron survival after nerve injury. Tissue Eng A 18(19–20):2030–2039
Fredriksson L, Li H, Fieber C, Li X, Eriksson U (2004) Tissue plasminogen activator is a potent activator of PDGF-CC. EMBO J 23(19):3793–3802
pubmed: 15372073
pmcid: 522796
García-Rocha M, Avila J, Armas-Portela R (1994) Tissue-type plasminogen activator (tPA) is the main plasminogen activator associated with isolated rat nerve growth cones. Neurosci Lett 180(2):123–126
pubmed: 7700563
Ghasemi-Mobarakeh L, Prabhakaran MP, Morshed M, Nasr-Esfahani M-H, Ramakrishna S (2008) Electrospun poly (ɛ-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials 29(34):4532–4539
pubmed: 18757094
Grinsell, D. and C. Keating (2014). Peripheral nerve reconstruction after injury: a review of clinical and experimental therapies. BioMed research international 2014
Gundersen H, Bagger P, Bendtsen T, Evans S, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard J, Pakkenberg B (1988a) The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. Apmis 96(7–12):857–881
pubmed: 3056461
Gundersen H, Bendtsen TF, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard J, Pakkenberg B, Sørensen FB, Vesterby A (1988b) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. Apmis 96(1–6):379–394
pubmed: 3288247
Himes B, Tessler A (1989) Death of some dorsal root ganglion neurons and plasticity of others following sciatic nerve section in adult and neonatal rats. J Comp Neurol 284(2):215–230
pubmed: 2474003
Hyun JK, Kim H-W (2010) Clinical and experimental advances in regeneration of spinal cord injury. Journal of tissue engineering 1(1):650857
Jensen EB, Gundersen HJG, Østerby R (1979) Determination of membrane thickness distribution from orthogonal intercepts. J Microsc 115(1):19–33
pubmed: 423237
Johnson EO, Charchanti A, Soucacos PN (2008) Nerve repair: experimental and clinical evaluation of neurotrophic factors in peripheral nerve regeneration. Injury 39(3):37–42
Karlsson LM, Cruz-Orive L (1997) Estimation of mean particle size from single sections. J Microsc 186(2):121–132
Kim CH, Khil MS, Kim HY, Lee HU, Jahng KY (2006) An improved hydrophilicity via electrospinning for enhanced cell attachment and proliferation. Journal of Biomedical Materials Research Part B: Applied Biomaterials: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials 78(2):283–290
Koh H, Yong T, Chan C, Ramakrishna S (2008) Enhancement of neurite outgrowth using nano-structured scaffolds coupled with laminin. Biomaterials 29(26):3574–3582
pubmed: 18533251
Larsen JO (1998) Stereology of nerve cross sections. J Neurosci Methods 85(1):107–118
pubmed: 9874147
Lee CH, Shin HJ, Cho IH, Kang Y-M, Kim IA, Park K-D, Shin J-W (2005) Nanofiber alignment and direction of mechanical strain affect the ECM production of human ACL fibroblast. Biomaterials 26(11):1261–1270
pubmed: 15475056
Li W-J, Cooper JA Jr, Mauck RL, Tuan RS (2006) Fabrication and characterization of six electrospun poly (α-hydroxy ester)-based fibrous scaffolds for tissue engineering applications. Acta Biomater 2(4):377–385
pubmed: 16765878
Mataga N, Nagai N, Hensch TK (2002) Permissive proteolytic activity for visual cortical plasticity. Proc Natl Acad Sci 99(11):7717–7721
pubmed: 12032349
Mayer M (1990) Biochemical and biological aspects of the plasminogen activation system. Clin Biochem 23(3):197–211
pubmed: 2197030
Noorafshan A, Omidi A, Karbalay-Doust S (2011) Curcumin protects the dorsal root ganglion and sciatic nerve after crush in rat. Pathology-Research and Practice 207(9):577–582
Noorafshan A, Hoseini L, Karbalay-Doust S, Nadimi E (2012) A simple stereological method for estimating the number and the volume of the pancreatic beta cells. JOP Journal of the Pancreas 13(4):427–432
pubmed: 22797400
Pierucci A, de Oliveira ALR (2006) Increased sensory neuron apoptotic death 2 weeks after peripheral axotomy in C57BL/6J mice compared to A/J mice. Neurosci Lett 396(2):127–131
pubmed: 16359790
Pittman RN, DiBenedetto AJ (1995) PC12 cells overexpressing tissue plasminogen activator regenerate neurites to a greater extent and migrate faster than control cells in complex extracellular matrix. J Neurochem 64(2):566–575
pubmed: 7830049
Prabhakaran MP, Venugopal J, Chan CK, Ramakrishna S (2008) Surface modified electrospun nanofibrous scaffolds for nerve tissue engineering. Nanotechnology 19(45):455102
pubmed: 21832761
Qian Z, Gilbert ME, Colicos MA, Kandel ER, Kuhl D (1993) Tissue-plasminogen activator is induced as an immediate–early gene during seizure, kindling and long-term potentiation. Nature 361(6411):453–457
pubmed: 8429885
Rogove AD, Siao C, Keyt B, Strickland S, Tsirka SE (1999) Activation of microglia reveals a non-proteolytic cytokine function for tissue plasminogen activator in the central nervous system. J Cell Sci 112(22):4007–4016
pubmed: 10547361
Schnell E, Klinkhammer K, Balzer S, Brook G, Klee D, Dalton P, Mey J (2007) Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-ε-caprolactone and a collagen/poly-ε-caprolactone blend. Biomaterials 28(19):3012–3025
pubmed: 17408736
Seeds NW, Williams BL, Bickford PC (1995) Tissue plasminogen activator induction in Purkinje neurons after cerebellar motor learning. Science 270(5244):1992–1994
pubmed: 8533091
Siconolfi LB, Seeds NW (2001) Mice lacking tPA, uPA, or plasminogen genes showed delayed functional recovery after sciatic nerve crush. J Neurosci 21(12):4348–4355
pubmed: 11404420
pmcid: 6762762
Siemionow M, Brzezicki G (2009) Current techniques and concepts in peripheral nerve repair. Int Rev Neurobiol 87:141–172
pubmed: 19682637
Soleimani M, Mahboudi F, Davoudi N, Amanzadeh A, Azizi M, Adeli A, Rastegar H, Barkhordari F, Mohajer-Maghari B (2007) Expression of human tissue plasminogen activator in the trypanosomatid protozoan Leishmania tarentolae. Biotechnol Appl Biochem 48(1):55–61
pubmed: 17371294
Terenghi G (1999) Peripheral nerve regeneration and neurotrophic factors. The Journal of Anatomy 194(1):1–14
pubmed: 10227662
Tsirka SE, Gualandris A, Amaral DG, Strickland S (1995) Excitotoxin-induced neuronal degeneration and seizure are mediated by tissue plasminogen activator. Nature 377(6547):340–344
pubmed: 7566088
Tsirka SE, Rogove AD, Bugge TH, Degen JL, Strickland S (1997) An extracellular proteolytic cascade promotes neuronal degeneration in the mouse hippocampus. J Neurosci 17(2):543–552
pubmed: 8987777
pmcid: 6573220
Varejão AS, Melo-Pinto P, Meek MF, Filipe VM, Bulas-Cruz J (2004) Methods for the experimental functional assessment of rat sciatic nerve regeneration. Neurol Res 26(2):186–194
pubmed: 15072638
Vassalli J-D, Sappino A, Belin D (1991) The plasminogen activator/plasmin system. J Clin Invest 88(4):1067–1072
pubmed: 1833420
pmcid: 295552
Vestergaard S, Tandrup T, Jakobsen J (1997) Effect of permanent axotomy on number and volume of dorsal root ganglion cell bodies. J Comp Neurol 388(2):307–312
pubmed: 9368843
Willerth SM, Sakiyama-Elbert SE (2007) Approaches to neural tissue engineering using scaffolds for drug delivery. Adv Drug Deliv Rev 59(4–5):325–338
pubmed: 17482308
pmcid: 1976339
Wu YP, Siao C-J, Lu W, Sung T-C, Frohman MA, Milev P, Bugge TH, Degen JL, Levine JM, Margolis RU (2000) The tissue plasminogen activator (tPA/plasmin) extracellular proteolytic system regulates seizure-induced hippocampal mossy fiber outgrowth through a proteoglycan substrate. J Cell Biol 148(6):1295–1304
pubmed: 10725341
pmcid: 2174310
Yang L, Fitie CF, van der Werf KO, Bennink ML, Dijkstra PJ, Feijen J (2008) Mechanical properties of single electrospun collagen type I fibers. Biomaterials 29(8):955–962
pubmed: 18082253
Yepes M, Sandkvist M, Coleman TA, Moore E, Wu J-Y, Mitola D, Bugge TH, Lawrence DA (2002) Regulation of seizure spreading by neuroserpin and tissue-type plasminogen activator is plasminogen-independent. J Clin Invest 109(12):1571–1578
pubmed: 12070304
pmcid: 151009
Yu W, Zhao W, Zhu C, Zhang X, Ye D, Zhang W, Zhou Y, Jiang X, Zhang Z (2011) Sciatic nerve regeneration in rats by a promising electrospun collagen/poly (ε-caprolactone) nerve conduit with tailored degradation rate. BMC Neurosci 12(1):68
pubmed: 21756368
pmcid: 3148572
Zalewski AA, Gulati AK (1981) Rejection of nerve allografts after cessation of immunosuppression with cyclosporin A. Transplantation 31(1):88–89
pubmed: 7233527