Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection.
Biomarkers, Tumor
Clinical Decision-Making
Diagnosis, Differential
Disease Management
Disease Susceptibility
Humans
Immunohistochemistry
/ instrumentation
Male
Microdissection
/ instrumentation
Molecular Diagnostic Techniques
/ methods
Neoplasms, Germ Cell and Embryonal
/ diagnosis
Testicular Neoplasms
/ diagnosis
Germ cell tumors
Laser capture microdissection
Molecular genetics
Testicular cancer
Tissue microdissection
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
28
8
2020
pubmed:
28
8
2020
medline:
25
3
2021
Statut:
ppublish
Résumé
Testicular germ cell tumors are among the most common malignancies seen in children and young adults. Genomic studies have identified characteristic molecular profiles in testicular cancer, which are associated with histologic subtypes and may predict clinical behavior including treatment responses. Emerging molecular technologies analyzing tumor genomics, transcriptomics, and proteomics may now guide precision management of testicular tumors. Laser-assisted microdissection methods such as laser capture microdissection efficiently isolate selected tumor cells from routine pathology specimens, avoiding contamination from nontarget cell populations. Laser capture microdissection in combination with next generation sequencing makes precise high throughput genetic evaluation effective and efficient. The use of laser capture microdissection (LCM) for molecular testing may translate into great benefits for the clinical management of patients with testicular cancers. This review discusses application protocols for laser-assisted microdissection to investigate testicular germ cell tumors.
Identifiants
pubmed: 32852755
doi: 10.1007/978-1-0716-0860-9_3
doi:
Substances chimiques
Biomarkers, Tumor
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
31-47Références
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