Cell culture media notably influence properties of human mesenchymal stroma/stem-like cells from different tissues.
Adipose Tissue
/ cytology
Antigens, Surface
/ metabolism
Biomarkers
/ metabolism
Bone Marrow Cells
/ cytology
Calcium
/ metabolism
Cell Culture Techniques
Cell Differentiation
/ drug effects
Cell Proliferation
/ drug effects
Cells, Cultured
Culture Media
/ pharmacology
Dental Pulp
/ cytology
Extracellular Vesicles
/ drug effects
Gene Expression Regulation
/ drug effects
Humans
Mesenchymal Stem Cells
/ cytology
Organ Specificity
/ drug effects
Osteogenesis
/ drug effects
Reproducibility of Results
Tetraspanins
/ metabolism
Tissue Donors
cell surface antigens
in vitro osteogenesis
mesenchymal stromal cells
morphology
population doubling time
tetraspanins
Journal
Cytotherapy
ISSN: 1477-2566
Titre abrégé: Cytotherapy
Pays: England
ID NLM: 100895309
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
31
03
2020
revised:
06
07
2020
accepted:
09
07
2020
pubmed:
29
8
2020
medline:
13
2
2021
entrez:
29
8
2020
Statut:
ppublish
Résumé
Mesenchymal stroma/stem-like cells (MSCs) are a popular cell source and hold huge therapeutic promise for a broad range of possible clinical applications. However, to harness their full potential, current limitations in harvesting, expansion and characterization have to be overcome. These limitations are related to the heterogeneity of MSCs in general as well as to inconsistent experimental protocols. Here we aim to compare in vitro methods to facilitate comparison of MSCs generated from various tissues. MSCs from 3 different tissues (bone marrow, dental pulp, adipose tissue), exemplified by cells from 3 randomly chosen donors per tissue, were systematically compared with respect to their in vitro properties after propagation in specific in-house standard media, as established in the individual laboratories, or in the same commercially available medium. Large differences were documented with respect to the expression of cell surface antigens, population doubling times, basal expression levels of 5 selected genes and osteogenic differentiation. The commercial medium reduced differences in these parameters with respect to individual human donors within tissue and between tissues. The extent, size and tetraspanin composition of extracellular vesicles were also affected. The results clearly demonstrate the extreme heterogeneity of MSCs, which confirms the problem of reproducibility of results, even when harmonizing experimental conditions, and questions the significance of common parameters for MSCs from different tissues in vitro.
Sections du résumé
BACKGROUND AIMS
Mesenchymal stroma/stem-like cells (MSCs) are a popular cell source and hold huge therapeutic promise for a broad range of possible clinical applications. However, to harness their full potential, current limitations in harvesting, expansion and characterization have to be overcome. These limitations are related to the heterogeneity of MSCs in general as well as to inconsistent experimental protocols. Here we aim to compare in vitro methods to facilitate comparison of MSCs generated from various tissues.
METHODS
MSCs from 3 different tissues (bone marrow, dental pulp, adipose tissue), exemplified by cells from 3 randomly chosen donors per tissue, were systematically compared with respect to their in vitro properties after propagation in specific in-house standard media, as established in the individual laboratories, or in the same commercially available medium.
RESULTS
Large differences were documented with respect to the expression of cell surface antigens, population doubling times, basal expression levels of 5 selected genes and osteogenic differentiation. The commercial medium reduced differences in these parameters with respect to individual human donors within tissue and between tissues. The extent, size and tetraspanin composition of extracellular vesicles were also affected.
CONCLUSIONS
The results clearly demonstrate the extreme heterogeneity of MSCs, which confirms the problem of reproducibility of results, even when harmonizing experimental conditions, and questions the significance of common parameters for MSCs from different tissues in vitro.
Identifiants
pubmed: 32855067
pii: S1465-3249(20)30792-1
doi: 10.1016/j.jcyt.2020.07.005
pii:
doi:
Substances chimiques
Antigens, Surface
0
Biomarkers
0
Culture Media
0
Tetraspanins
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
653-668Informations de copyright
Copyright © 2020 International Society for Cell & Gene Therapy. Published by Elsevier Inc. All rights reserved.