Engineered Ferritin Nanoparticles for the Bioluminescence Tracking of Nanodrug Delivery in Cancer.

apoferritin nanoparticles luciferase/luciferin nanomedicine self-immolative linkers tumor targeting

Journal

Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338

Informations de publication

Date de publication:
10 2020
Historique:
received: 05 03 2020
revised: 16 07 2020
pubmed: 29 8 2020
medline: 4 5 2021
entrez: 29 8 2020
Statut: ppublish

Résumé

The identification of a highly sensitive method to check the delivery of administered nanodrugs into the tumor cells is a crucial step of preclinical studies aimed to develop new nanoformulated cures, since it allows the real therapeutic potential of these devices to be forecast. In the present work, the ability of an H-ferritin (HFn) nanocage, already investigated as a powerful tool for cancer therapy thanks to its ability to actively interact with the transferrin receptor 1, to act as an efficient probe for the monitoring of nanodrug delivery to tumors is demonstrated. The final formulation is a bioluminescent nanoparticle, where the luciferin probe is conjugated on nanoparticle surface by means of a disulfide containing linker (Luc-linker@HFn) which is subjected to glutathione-induced cyclization in tumor cell cytoplasm. The prolonged imaging of luciferase+ tumor models, demonstrated by an in vitro and an in vivo approach, associated with the prolonged release of luciferin into cancer cells by disulfide bridge reduction, clearly indicates the high efficiency of Luc-linker@HFn for drug delivery to the tumor tissues.

Identifiants

pubmed: 32856404
doi: 10.1002/smll.202001450
doi:

Substances chimiques

Apoferritins 9013-31-4

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2001450

Informations de copyright

© 2020 Wiley-VCH GmbH.

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Auteurs

Michela Bellini (M)

Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, Milan, 20126, Italy.

Benedetta Riva (B)

Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, Milan, 20126, Italy.

Veronica Tinelli (V)

Nanomedicine laboratory, ICS Maugeri S.p.A. SB, via S. Maugeri 10, Pavia, 27100, Italy.

Maria Antonietta Rizzuto (MA)

Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, Milan, 20126, Italy.

Lucia Salvioni (L)

Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, Milan, 20126, Italy.

Miriam Colombo (M)

Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, Milan, 20126, Italy.

Francesca Mingozzi (F)

Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, Milan, 20126, Italy.

Alberto Visioli (A)

STEMGEN Spa, c/o Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, Milan, 20126, Italy.

Laura Marongiu (L)

Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, Milan, 20126, Italy.

Gianni Frascotti (G)

Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, Milan, 20126, Italy.

Michael S Christodoulou (MS)

Department of Chemistry, University of Milan, via Golgi 19, Milan, 20133, Italy.

Daniele Passarella (D)

Department of Chemistry, University of Milan, via Golgi 19, Milan, 20133, Italy.

Davide Prosperi (D)

Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, Milan, 20126, Italy.
Nanomedicine laboratory, ICS Maugeri S.p.A. SB, via S. Maugeri 10, Pavia, 27100, Italy.

Luisa Fiandra (L)

Department of Earth and Environmental Sciences, University of Milano-Bicocca, P.zza della Scienza 1, Milan, 20126, Italy.

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