LncRNA NCK1-AS1 promotes non-small cell lung cancer progression via regulating miR-512-5p/p21 axis.
Adaptor Proteins, Signal Transducing
/ genetics
Adult
Aged
Carcinoma, Non-Small-Cell Lung
/ genetics
Cell Movement
/ genetics
Cell Proliferation
/ genetics
Disease Progression
Female
Gene Expression Regulation, Neoplastic
/ genetics
Humans
Lung Neoplasms
/ genetics
Male
MicroRNAs
/ genetics
Middle Aged
Oncogene Proteins
/ genetics
RNA, Antisense
/ genetics
RNA, Long Noncoding
/ genetics
Invasion
MiR-512-5p
Migration
NCK1-AS1
NSCLC
Proliferation
Journal
Pathology, research and practice
ISSN: 1618-0631
Titre abrégé: Pathol Res Pract
Pays: Germany
ID NLM: 7806109
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
10
03
2020
revised:
29
07
2020
accepted:
01
08
2020
pubmed:
29
8
2020
medline:
26
8
2021
entrez:
29
8
2020
Statut:
ppublish
Résumé
This study aimed at probing into the effect of lncRNA NCK1-AS1 on proliferation, migration and invasion of non-small cell lung cancer (NSCLC) cells and its regulatory function on miR-512-5p/p21 molecular axis. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to assess the expressions of NCK1-AS1 and miR-512-5p in NSCLC tissues and cell lines. The alterations of cell proliferation, migration, invasion and cell cycle were examined by cell counting kit-8 (CCK-8) assay, BrdU experiment, Transwell experiment and flow cytometry, respectively. The dual-luciferase reporter assay and RNA immunoprecipitation experiment were performed to validate the binding relationships between miR-512-5p and NCK1-AS1, and miR-512-5p the 3'UTR of p21 mRNA. Western blot was used to determine the effects of NCK1-AS1 and miR-512-5p on p21 protein expression. NCK1-AS1 expression was up-regulated in NSCLC tissues and cells, and its high expression was correlated with shorter overall survival time and faster progression of patients. Overexpression of NCK1-AS1 promoted NSCLC cell proliferation, migration and invasion, and accelerated the cell cycle, whereas NCK1-AS1 siRNA inhibited these malignant biological behaviors, and arrested cell cycle. NCK1-AS1 could bind to miR-512-5p, p21 was verified as a target gene of miR-512-5p, and NCK1-AS1 could up-regulate the expression of p21 in NSCLC cells via repressing miR-512-5p expression. NCK1-AS1 promotes NSCLC progression by regulating miR-512-5p/p21 molecular axis.
Identifiants
pubmed: 32858372
pii: S0344-0338(20)32012-4
doi: 10.1016/j.prp.2020.153157
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
MIRN512 microRNA, human
0
MicroRNAs
0
Nck protein
0
Oncogene Proteins
0
RNA, Antisense
0
RNA, Long Noncoding
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
153157Informations de copyright
Copyright © 2020 Elsevier GmbH. All rights reserved.