Selective flexible packaging pathways of the segmented genome of influenza A virus.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 08 2020
28 08 2020
Historique:
received:
12
11
2019
accepted:
30
07
2020
entrez:
30
8
2020
pubmed:
30
8
2020
medline:
18
9
2020
Statut:
epublish
Résumé
The genome of influenza A viruses (IAV) is encoded in eight distinct viral ribonucleoproteins (vRNPs) that consist of negative sense viral RNA (vRNA) covered by the IAV nucleoprotein. Previous studies strongly support a selective packaging model by which vRNP segments are bundling to an octameric complex, which is integrated into budding virions. However, the pathway(s) generating a complete genome bundle is not known. We here use a multiplexed FISH assay to monitor all eight vRNAs in parallel in human lung epithelial cells. Analysis of 3.9 × 10
Identifiants
pubmed: 32859915
doi: 10.1038/s41467-020-18108-1
pii: 10.1038/s41467-020-18108-1
pmc: PMC7455735
doi:
Substances chimiques
RNA, Viral
0
Ribonucleoproteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4355Références
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