A puzzle piece of protein N-glycosylation in chicken egg: N-glycoproteome of chicken egg vitelline membrane.

Animals Chickens Chromatography, Liquid Egg Proteins / analysis Gene Ontology Glycoproteins / analysis Mucin-5B / metabolism Tandem Mass Spectrometry Vitelline Membrane / metabolism
Chicken egg vitelline membrane N-glycoproteome Transmembrane transport of lipids

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
01 Dec 2020
Historique:
received: 13 07 2020
revised: 11 08 2020
accepted: 24 08 2020
pubmed: 30 8 2020
medline: 13 4 2021
entrez: 30 8 2020
Statut: ppublish

Résumé

The chicken egg vitelline membrane (CEVM) is an important structure for the transmembrane transport of egg yolk components, protection of the blastodisc, and separation of egg white and egg yolk. In this study, the N-glycoproteome of the CEVM was mapped and analyzed in depth. Total protein of the CEVM was digested, and the glycopeptides were enriched by a hydrophilic interaction liquid chromatography microcolumn and identified by nano liquid chromatography/tandem mass spectrometry. A total of 435 N-glycosylation sites on 208 N-glycoproteins were identified in CEVM. Gene Ontology enrichment analysis showed that CEVM N-glycoproteins are mainly involved in the regulation of proteinases/inhibitors and transmembrane transport of lipids. Mucin-5B is the primary N-glycoprotein in the CEVM. Comparison of the main N-glycoproteins between the CEVM and other egg parts revealed the tissue specificity of N-glycosylation of egg proteins. The results provide insights into protein N-glycosylation in the chicken egg, CEVM functions and underlying mechanisms.

Identifiants

DOI: 10.1016/j.ijbiomac.2020.08.193 PMID: 32860793 PMC: PMC7448747
pubmed: 32860793
pii: S0141-8130(20)34291-4
doi: 10.1016/j.ijbiomac.2020.08.193
pmc: PMC7448747
pii:
doi:

Substances chimiques

Egg Proteins 0
Glycoproteins 0
Mucin-5B 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

3125-3132

Informations de copyright

Copyright © 2020. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no competing financial interests.

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Auteurs

Jing Xiao (J)

Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China.

Jinqiu Wang (J)

Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China.

Lei Cheng (L)

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China. Electronic address: chenglei@btbu.edu.cn.

Sihai Gao (S)

Department of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Electronic address: sihaigao73@163.com.

Shugang Li (S)

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.

Ning Qiu (N)

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Hanmei Li (H)

Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China.

Lianxin Peng (L)

Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China.

Fang Geng (F)

Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China. Electronic address: gengfang@cdu.edu.cn.

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Classifications MeSH

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questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides salivaires › Mucine 5B › A puzzle piece of protein N-glycosylation in...
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