A bioenergetic shift is required for spermatogonial differentiation.
Cell biology
Stem cells
Journal
Cell discovery
ISSN: 2056-5968
Titre abrégé: Cell Discov
Pays: England
ID NLM: 101661034
Informations de publication
Date de publication:
2020
2020
Historique:
received:
04
02
2020
accepted:
22
05
2020
entrez:
1
9
2020
pubmed:
31
8
2020
medline:
31
8
2020
Statut:
epublish
Résumé
A bioenergetic balance between glycolysis and mitochondrial respiration is particularly important for stem cell fate specification. It however remains to be determined whether undifferentiated spermatogonia switch their preference for bioenergy production during differentiation. In this study, we found that ATP generation in spermatogonia was gradually increased upon retinoic acid (RA)-induced differentiation. To accommodate this elevated energy demand, RA signaling concomitantly switched ATP production in spermatogonia from glycolysis to mitochondrial respiration, accompanied by increased levels of reactive oxygen species. Disrupting mitochondrial respiration significantly blocked spermatogonial differentiation. Inhibition of glucose conversion to glucose-6-phosphate or pentose phosphate pathway also repressed the formation of c-Kit
Identifiants
pubmed: 32864161
doi: 10.1038/s41421-020-0183-x
pii: 183
pmc: PMC7431567
doi:
Types de publication
Journal Article
Langues
eng
Pagination
56Subventions
Organisme : Ministry of Science and Technology of the People's Republic of China (Chinese Ministry of Science and Technology)
ID : 2016YFA0100300
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 91854123
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 31771655
Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Conflict of interestThe authors declare that they have no conflict of interest.
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