The Sperm Proteome of the Oyster Crassostrea hongkongensis.

Animals Chromatography, Liquid Crassostrea Male Proteome Proteomics Spermatozoa / physiology Tandem Mass Spectrometry
Bivalvia bindin oyster proteomics sperm proteome

Journal

Proteomics
ISSN: 1615-9861
Titre abrégé: Proteomics
Pays: Germany
ID NLM: 101092707

Informations de publication

Date de publication:
10 2020
Historique:
received: 22 06 2020
revised: 13 08 2020
pubmed: 1 9 2020
medline: 1 9 2021
entrez: 1 9 2020
Statut: ppublish

Résumé

Sperm proteins play vital roles in fertilization, but little is known about their identities in free-spawning marine invertebrates. Here, 286 sperm proteins are reported from the Hong Kong oyster Crassostrea hongkongensis using label-free and semi-quantitative proteomics. Proteins extracted from three sperm samples are separated by SDS-PAGE, analyzed by LC-MS/MS, and identified using Mascot. Functional classification of the sperm proteome reveals energy metabolism (33%), signaling and binding (23%), and protein synthesis and degradation (12%) as the top functional categories. Comparison of orthologous sperm proteins between C. hongkongensis, Crassostrea gigas, Mytilus edulis, and M. galloprovincialis suggests that energy metabolism (48%) is the most conserved functional group. Sequence alignment of the C. hongkongensis bindin, an acrosomal protein that binds the sperm and the egg, with those of three other Crassostrea species, reveals several conserved motifs. The study has enriched the data of invertebrate sperm proteins and may contribute to studies of mechanisms of fertilization in free-spawning invertebrates. The proteomic data are available in ProteomeXchange with the identifier PXD018255.

Identifiants

DOI: 10.1002/pmic.202000167 PMID: 32865869
pubmed: 32865869
doi: 10.1002/pmic.202000167
doi:

Substances chimiques

Proteome 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2000167

Informations de copyright

© 2020 Wiley-VCH GmbH.

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Auteurs

Huawei Mu (H)

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230026, China.

Shengwei Ke (S)

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230026, China.

Duo Zhang (D)

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230026, China.

Yanjie Zhang (Y)

Department of Biology and Hong Kong Branch of the Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Hong Kong Baptist University, Hong Kong, China.

Xiaoyuan Song (X)

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230026, China.

Ziniu Yu (Z)

Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.

Yang Zhang (Y)

Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.

Jian-Wen Qiu (JW)

Department of Biology and Hong Kong Branch of the Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Hong Kong Baptist University, Hong Kong, China.
ORCID: 0000-0002-1541-9627

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › The Sperm Proteome of the Oyster Crassostrea...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › The Sperm Proteome of the Oyster Crassostrea...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Mollusca › Bivalvia › Ostreidae › Crassostrea › The Sperm Proteome of the Oyster Crassostrea...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéome › The Sperm Proteome of the Oyster Crassostrea...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › The Sperm Proteome of the Oyster Crassostrea...
questionsmedicales.fr › Cellules › Cellules germinales › Spermatozoïdes › The Sperm Proteome of the Oyster Crassostrea...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › The Sperm Proteome of the Oyster Crassostrea...