Human γδ T cells recognize CD1b by two distinct mechanisms.


Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
15 09 2020
Historique:
pubmed: 2 9 2020
medline: 28 10 2020
entrez: 2 9 2020
Statut: ppublish

Résumé

γδ T cells form an abundant part of the human cellular immune system, where they respond to tissue damage, infection, and cancer. The spectrum of known molecular targets recognized by Vδ1-expressing γδ T cells is becoming increasingly diverse. Here we describe human γδ T cells that recognize CD1b, a lipid antigen-presenting molecule, which is inducibly expressed on monocytes and dendritic cells. Using CD1b tetramers to study multiple donors, we found that many CD1b-specific γδ T cells use Vδ1. Despite their common use of Vδ1, three CD1b-specific γδ T cell receptors (TCRs) showed clear differences in the surface of CD1b recognized, the requirement for lipid antigens, and corecognition of butryophilin-like proteins. Several Vγ segments were present among the CD1b-specific TCRs, but chain swap experiments demonstrated that CD1b specificity was mediated by the Vδ1 chain. One of the CD1b-specific Vδ1

Identifiants

pubmed: 32868441
pii: 2010545117
doi: 10.1073/pnas.2010545117
pmc: PMC7502712
doi:

Substances chimiques

Antigens, CD1 0
CD1b antigen 0
Lipids 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

22944-22952

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : R01 AI049313
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR048632
Pays : United States
Organisme : Wellcome Trust
ID : 106292/Z/14/Z
Pays : United Kingdom

Déclaration de conflit d'intérêts

The authors declare no competing interest.

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Auteurs

Josephine F Reijneveld (JF)

Division of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital, Boston, MA 02115.
Department of Medicine, Harvard Medical School, Boston, MA 02115.
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.
Stratingh Institute for Chemistry, University of Groningen, 9747AG Groningen, The Netherlands.

Tonatiuh A Ocampo (TA)

Division of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital, Boston, MA 02115.
Department of Medicine, Harvard Medical School, Boston, MA 02115.

Adam Shahine (A)

Infection and Immunity Program, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia.
Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia.
Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Monash University, Clayton, VIC 3800, Australia.

Benjamin S Gully (BS)

Infection and Immunity Program, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia.
Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia.
Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Monash University, Clayton, VIC 3800, Australia.

Pierre Vantourout (P)

Peter Gorer Department of Immunobiology, School of Immunology & Microbial Sciences, King's College London, SE1 9RT London, United Kingdom.
Immunosurveillance Laboratory, The Francis Crick Institute, NW1 1AT London, United Kingdom.

Adrian C Hayday (AC)

Peter Gorer Department of Immunobiology, School of Immunology & Microbial Sciences, King's College London, SE1 9RT London, United Kingdom.
Immunosurveillance Laboratory, The Francis Crick Institute, NW1 1AT London, United Kingdom.

Jamie Rossjohn (J)

Infection and Immunity Program, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia.
Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia.
Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Monash University, Clayton, VIC 3800, Australia.
Institute of Infection and Immunity, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, United Kingdom.

D Branch Moody (DB)

Division of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital, Boston, MA 02115.
Department of Medicine, Harvard Medical School, Boston, MA 02115.

Ildiko Van Rhijn (I)

Division of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital, Boston, MA 02115; i.vanrhijn@uu.nl.
Department of Medicine, Harvard Medical School, Boston, MA 02115.
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands.

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Classifications MeSH