A drug screening toolkit based on the -1 ribosomal frameshifting of SARS-CoV-2.
-1 ribosomal frameshifting
Biomedical engineering
Drug screen
Microbial biotechnology
Molecular biology
Peptides
Protein engineering
SARS-Cov-2
Virology
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
14
07
2020
revised:
17
08
2020
accepted:
24
08
2020
entrez:
2
9
2020
pubmed:
2
9
2020
medline:
2
9
2020
Statut:
ppublish
Résumé
The -1 ribosomal frameshifting is vital for the translation of the open reading frame (ORF)1b in SARS-CoV-2. The products of ORF1b participate in viral replication. Therefore, changing the frameshift frequency reduces the survival of the virus. This study aimed to successfully develop a toolkit for screening antiviral drugs. Finally, the FDA-approved drug library was screened, revealing that ivacaftor and (-)-Huperzine A worked well in changing the -1 ribosomal frameshifting of SARS-CoV-2
Identifiants
pubmed: 32869005
doi: 10.1016/j.heliyon.2020.e04793
pii: S2405-8440(20)31636-4
pii: e04793
pmc: PMC7448739
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e04793Informations de copyright
© 2020 Published by Elsevier Ltd.
Références
J Virol. 2020 Oct 14;94(21):
pubmed: 32817221
Infect Disord Drug Targets. 2009 Apr;9(2):223-45
pubmed: 19275708
Cell. 2020 May 14;181(4):914-921.e10
pubmed: 32330414
J Biol Chem. 2020 Jul 31;295(31):10741-10748
pubmed: 32571880
Pharmacol Res. 2020 Sep;159:104960
pubmed: 32473310
Nucleic Acids Res. 2005 Jul 29;33(13):4265-75
pubmed: 16055920
Science. 2020 Jun 26;368(6498):1422-1424
pubmed: 32461212
Gene. 1991 Jul 22;103(2):171-7
pubmed: 1889744
Nucleic Acids Res. 2004 Feb 03;32(2):784-90
pubmed: 14762205
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
Antiviral Res. 2011 Jul;91(1):1-10
pubmed: 21549154
Proc Natl Acad Sci U S A. 2020 Jun 30;117(26):15075-15084
pubmed: 32532919
Virology. 2005 Feb 20;332(2):498-510
pubmed: 15680415
Cell. 2019 Jan 24;176(3):625-635.e14
pubmed: 30682371
Wiley Interdiscip Rev RNA. 2012 Sep-Oct;3(5):661-73
pubmed: 22715123
Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11133-8
pubmed: 12149516
J Am Chem Soc. 2011 Jul 6;133(26):10094-100
pubmed: 21591761
J Virol. 2010 May;84(9):4330-40
pubmed: 20164235