Extracellular vesicles on demand (EVOD) chip for screening and quantification of cancer-associated extracellular vesicles.
Click chemistry
Extracellular vesicles
Immunoaffinity isolation
Microfluidics
Vesicle release
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
15 Nov 2020
15 Nov 2020
Historique:
received:
28
06
2020
revised:
04
08
2020
accepted:
20
08
2020
pubmed:
2
9
2020
medline:
15
5
2021
entrez:
2
9
2020
Statut:
ppublish
Résumé
While significant advancements have been made in cancer therapeutics and treatments, early disease detection and diagnosis remains critical to ensuring favorable outcomes for patients. To that end, we propose a microfluidic based approach to the sensitive detection of an intriguing cancer biomarker, extracellular vesicles (EVs). Our extracellular vesicles on demand (EVOD) chip utilizes a catalyst-free click chemistry to rapidly and specifically isolate EVs of interest. This specific isolation is followed by subsequent dithiothreitol release of the isolated EVs for downstream functional analysis. This joint isolation and release provide a powerful tool for the screening and quantification of EVs of interest. By incorporating antibodies against cancer associated surface proteins into the click-chemistry, we were able to selectively recover cancer-associated exosomes, allowing for important insights into patient disease. This platform was also tested using non-small cell lung cancer (NSCLC) patient samples, where anti-epidermal growth factor receptor (EGFR) assisted platform were able to selectively isolate and release 76% more exosomes from NSCLC patients than from healthy donors. This matches the previously reported higher EGFR expression commonly found in NSCLC EVs. Through its rapid isolation kinetics and adaptability in marker targeting, the EVOD device provides a highly versatile liquid biopsy platform for clinicians to use in the fight against cancer.
Identifiants
pubmed: 32871498
pii: S0956-5663(20)30527-3
doi: 10.1016/j.bios.2020.112535
pmc: PMC9475444
mid: NIHMS1626704
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112535Subventions
Organisme : NCI NIH HHS
ID : R01 CA208335
Pays : United States
Organisme : NCI NIH HHS
ID : R33 CA202867
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
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