Salivary
Biomarkers
cancer
early detection
nuclear undecaprenyl pyrophosphate synthase 1(NUS1)
oral squamous cell carcinoma
reticulocalbin 1(RCN1)
Journal
In vivo (Athens, Greece)
ISSN: 1791-7549
Titre abrégé: In Vivo
Pays: Greece
ID NLM: 8806809
Informations de publication
Date de publication:
Historique:
received:
08
04
2020
revised:
13
05
2020
accepted:
25
05
2020
entrez:
3
9
2020
pubmed:
3
9
2020
medline:
22
6
2021
Statut:
ppublish
Résumé
Oral cancer may become advanced because of delay in diagnosis. In order to promote oral cancer screening, simple and highly reliable screening methods that can be implemented at general dental clinics are required. Herein we investigated differential salivary gene expression between oral squamous cell carcinoma (OSCC) patients and healthy volunteers (HV) to identify new biomarkers for OSCC detection. Candidate genes were selected by microarrays, nuclear undecaprenyl pyrophosphate synthase 1 (NUS1) and reticulocalbin 1 (RCN1) were selected for further investigation. We used real-time quantitative reverse transcription PCR (qRT-PCR) to determine NUS1 and RCN1 expression levels in saliva and tissues. qRT-PCR analysis of clinical samples revealed that OSCC patients had significantly higher expression of salivary NUS1 and RCN1 than HV. A combination of NUS1 and RCN1 accurately distinguished patients from controls, and this combination can be implemented as a screening test for OSCC.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
Oral cancer may become advanced because of delay in diagnosis. In order to promote oral cancer screening, simple and highly reliable screening methods that can be implemented at general dental clinics are required. Herein we investigated differential salivary gene expression between oral squamous cell carcinoma (OSCC) patients and healthy volunteers (HV) to identify new biomarkers for OSCC detection.
MATERIALS AND METHODS
METHODS
Candidate genes were selected by microarrays, nuclear undecaprenyl pyrophosphate synthase 1 (NUS1) and reticulocalbin 1 (RCN1) were selected for further investigation. We used real-time quantitative reverse transcription PCR (qRT-PCR) to determine NUS1 and RCN1 expression levels in saliva and tissues.
RESULTS
RESULTS
qRT-PCR analysis of clinical samples revealed that OSCC patients had significantly higher expression of salivary NUS1 and RCN1 than HV.
CONCLUSION
CONCLUSIONS
A combination of NUS1 and RCN1 accurately distinguished patients from controls, and this combination can be implemented as a screening test for OSCC.
Identifiants
pubmed: 32871760
pii: 34/5/2353
doi: 10.21873/invivo.12048
pmc: PMC7652505
doi:
Substances chimiques
Biomarkers
0
Biomarkers, Tumor
0
Calcium-Binding Proteins
0
NUS1 protein, human
0
RCN1 protein, human
0
Receptors, Cell Surface
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2353-2361Informations de copyright
Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
Références
Oncogene. 2017 Jun 15;36(24):3406-3416
pubmed: 28068323
Oncotarget. 2016 Feb 23;7(8):8850-65
pubmed: 26840457
J Proteomics. 2015 Jan 1;112:38-52
pubmed: 25173099
J Biol Chem. 1993 Jan 5;268(1):699-705
pubmed: 8416973
Hum Pathol. 2019 Jan;83:140-148
pubmed: 30172915
Electrophoresis. 2001 Aug;22(14):2990-3000
pubmed: 11565793
CA Cancer J Clin. 2016 Jan-Feb;66(1):7-30
pubmed: 26742998
Biochem Biophys Res Commun. 1997 Feb 13;231(2):283-9
pubmed: 9070264
J Neurosci. 2002 May 1;22(9):3553-67
pubmed: 11978832
Anticancer Res. 2020 May;40(5):2467-2474
pubmed: 32366390
Clin Transl Med. 2014 Feb 24;3(1):3
pubmed: 24564868
Anticancer Res. 2014 Sep;34(9):4819-28
pubmed: 25202063
J Proteomics. 2013 Oct 8;91:385-92
pubmed: 23916412
J Cell Biochem. 2008 Aug 15;104(6):2298-309
pubmed: 18561328
J Histochem Cytochem. 2007 Apr;55(4):335-45
pubmed: 17189526
Acta Odontol Scand. 2016;74(3):170-7
pubmed: 26577643
Electrophoresis. 2000 Aug;21(14):3058-68
pubmed: 11001323
DNA Cell Biol. 2017 Dec;36(12):1142-1150
pubmed: 29058484
Cardiovasc Res. 2003 Aug 1;59(2):400-11
pubmed: 12909323
Cancer Lett. 2018 Apr 1;418:135-146
pubmed: 29331415
J Oral Maxillofac Surg. 2003 Mar;61(3):285-91
pubmed: 12618965
Br J Oral Maxillofac Surg. 2005 Aug;43(4):281-4
pubmed: 15993279
PLoS One. 2018 Jan 18;13(1):e0191371
pubmed: 29346419
Proc Natl Acad Sci U S A. 2006 Jul 18;103(29):10997-1002
pubmed: 16835300
J Clin Oncol. 2015 Oct 10;33(29):3269-76
pubmed: 26351335
Anticancer Res. 2017 Jul;37(7):3473-3481
pubmed: 28668836
Cancer Lett. 2000 Nov 10;160(1):37-43
pubmed: 11098082
PLoS One. 2013 Nov 04;8(11):e78083
pubmed: 24223763
Cancer Lett. 2018 Apr 10;419:233-244
pubmed: 29373839
BMC Cancer. 2018 Apr 18;18(1):439
pubmed: 29669525
Gerodontology. 2013 Sep;30(3):194-200
pubmed: 22607478
Anticancer Res. 2016 Jul;36(7):3401-7
pubmed: 27354599