Microbiome Patterns in Matched Bile, Duodenal, Pancreatic Tumor Tissue, Drainage, and Stool Samples: Association with Preoperative Stenting and Postoperative Pancreatic Fistula Development.
16S rRNA sequencing
microbiome
pancreatic cancer
pancreatic surgery
postoperative complications
Journal
Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588
Informations de publication
Date de publication:
28 Aug 2020
28 Aug 2020
Historique:
received:
03
07
2020
revised:
10
08
2020
accepted:
24
08
2020
entrez:
3
9
2020
pubmed:
3
9
2020
medline:
3
9
2020
Statut:
epublish
Résumé
Postoperative complications after pancreatic surgery are still a significant problem in clinical practice. The aim of this study was to characterize and compare the microbiomes of different body compartments (bile duct, duodenal mucosa, pancreatic tumor lesion, postoperative drainage fluid, and stool samples; preoperative and postoperative) in patients undergoing pancreatic surgery for suspected pancreatic cancer, and their association with relevant clinical factors (stent placement, pancreatic fistula, and gland texture). For this, solid (duodenal mucosa, pancreatic tumor tissue, stool) and liquid (bile, drainage fluid) biopsy samples of 10 patients were analyzed using 16s rRNA gene next-generation sequencing. Our analysis revealed: (i) a distinct microbiome in the different compartments, (ii) markedly higher abundance of
Identifiants
pubmed: 32872220
pii: jcm9092785
doi: 10.3390/jcm9092785
pmc: PMC7563524
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Tumorzentrum Erlangen
ID : 2019
Références
Cell. 2019 Aug 8;178(4):795-806.e12
pubmed: 31398337
Sci Rep. 2019 Nov 14;9(1):16801
pubmed: 31727922
BMC Microbiol. 2019 Feb 18;19(1):42
pubmed: 30777006
J Gastrointest Surg. 2014 Jan;18(1):172-79; discussion 179-80
pubmed: 24002771
Trends Cancer. 2020 Mar;6(3):192-204
pubmed: 32101723
World J Surg. 2008 Mar;32(3):419-28
pubmed: 18175170
World J Gastrointest Oncol. 2019 Sep 15;11(9):717-728
pubmed: 31558976
Gut. 2020 Apr;69(4):665-672
pubmed: 31243055
Nat Protoc. 2020 Mar;15(3):799-821
pubmed: 31942082
PLoS One. 2017 Aug 10;12(8):e0182924
pubmed: 28796833
Gut. 2019 Mar;68(3):578-580
pubmed: 29581241
Cancer Discov. 2018 Apr;8(4):403-416
pubmed: 29567829
Carcinogenesis. 2020 Jun 17;41(4):397-404
pubmed: 32034405
Nat Med. 2019 Apr;25(4):679-689
pubmed: 30936547
Visc Med. 2017 May;33(2):126-130
pubmed: 28560227
Mol Cancer. 2019 May 20;18(1):97
pubmed: 31109338
Cancer Cell. 2019 Dec 9;36(6):577-579
pubmed: 31951558
BMC Surg. 2019 Aug 13;19(1):108
pubmed: 31409334
Br J Surg. 2018 Sep;105(10):1368-1376
pubmed: 29658991
JAMA Oncol. 2018 Nov 1;4(11):1553-1568
pubmed: 29860482
Nat Methods. 2013 Oct;10(10):996-8
pubmed: 23955772
Sci Transl Med. 2015 May 6;7(286):286ra68
pubmed: 25947163
Microbiome. 2014 Feb 24;2(1):6
pubmed: 24558975
Nat Rev Gastroenterol Hepatol. 2020 Jan;17(1):53-64
pubmed: 31811279
Cancer Cell. 2018 Apr 9;33(4):570-580
pubmed: 29634945
Bioinformatics. 2010 Oct 1;26(19):2460-1
pubmed: 20709691
World J Surg. 2014 Nov;38(11):2967-72
pubmed: 24952079
Ann Oncol. 2015 Sep;26 Suppl 5:v56-68
pubmed: 26314780
JAMA Surg. 2017 Nov 1;152(11):1030
pubmed: 28700806
Nat Rev Cancer. 2019 Jul;19(7):371-376
pubmed: 31186547
Genome Biol. 2011 Jun 24;12(6):R60
pubmed: 21702898
Cancers (Basel). 2020 Apr 25;12(5):
pubmed: 32344895
World J Gastroenterol. 2019 Nov 7;25(41):6238-6247
pubmed: 31749594
J Am Coll Surg. 2013 Jan;216(1):1-14
pubmed: 23122535
Nucleic Acids Res. 2017 Jul 3;45(W1):W180-W188
pubmed: 28449106