Sarkosyl Preparation of Antigens from Bacterial Inclusion Bodies.
Journal
Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530
Informations de publication
Date de publication:
01 09 2020
01 09 2020
Historique:
entrez:
3
9
2020
pubmed:
3
9
2020
medline:
30
7
2021
Statut:
epublish
Résumé
In many cases, solubility and proper folding of fusion proteins expressed in bacteria pose a major challenge in protein purification and crystallization. This is especially true when the fusion proteins are of eukaryotic origin. They form aggregates or become packaged into inclusion bodies, which makes protein purification extremely difficult. Sarkosyl is widely used to extract misfolded proteins from inclusion bodies in soluble form.
Identifiants
pubmed: 32873728
pii: 2020/9/pdb.prot100032
doi: 10.1101/pdb.prot100032
doi:
Substances chimiques
Antigens, Bacterial
0
Recombinant Fusion Proteins
0
sarkosyl
632GS99618
Sarcosine
Z711V88R5F
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
100032Informations de copyright
© 2020 Cold Spring Harbor Laboratory Press.