Induction of tenogenic differentiation of equine adipose-derived mesenchymal stem cells by platelet-derived growth factor-BB and growth differentiation factor-6.

Animals Basic Helix-Loop-Helix Transcription Factors / genetics Becaplermin / pharmacology Cell Differentiation / genetics Collagen Type I / genetics Female Gene Expression Regulation, Developmental / drug effects Growth Differentiation Factor 6 / pharmacology Homeodomain Proteins / genetics Horses Mesenchymal Stem Cells / metabolism Real-Time Polymerase Chain Reaction Tendons / cytology Tissue Engineering / methods
Equine Growth factors Mesechymal stem cells Tendon repair Tenogenic markers

Journal

Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234

Informations de publication

Date de publication:
Sep 2020
Historique:
received: 27 06 2020
accepted: 27 08 2020
revised: 15 08 2020
pubmed: 3 9 2020
medline: 4 6 2021
entrez: 3 9 2020
Statut: ppublish

Résumé

Managing tendon healing process is complicated mainly due to the limited regeneration capacity of tendon tissue. Mesenchymal stem cells (MSCs) have potential applications in regenerative medicine and have been considered for tendon repair and regeneration. This study aimed to evaluate the capacity of equine adipose tissue-derived cells (eASCs) to differentiate into tenocytes in response to platelet-derived growth factor-BB (PDGF-BB) and growth differentiation factor-6 (GDF-6) in vitro. Frozen characterized eASCS of 3 mares were thawed and the cells were expanded in basic culture medium (DMEM supplemented with 10% FBS). The cells at passage 5 were treated for 14 days in different conditions including: (1) control group in basic culture medium (CM), (2) induction medium as IM (CM containing L-prolin, and ascorbic acid (AA)) supplemented with PDGF-BB (20 ng/ml), (3) IM supplemented with GDF-6 (20 ng/ml), and (4) IM supplemented with PDGF-BB and GDF-6. At the end of culture period (14th day), tenogenic differentiation was evaluated. Sirius Red staining was used to assess collagen production, and H&E was used for assessing cell morphology. mRNA levels of collagen type 1 (colI), scleraxis (SCX), and Mohawk (MKX), as tenogenic markers, were analyzed using real-time reverse-transcription polymerase chain reaction (qPCR). H&E staining showed a stretching and spindle shape (tenocyte-like) cells in all treated groups compared to unchanged from of cells in control groups. Also, Sirius red staining data showed a significant increase in collagen production in all treated groups compared with the control group. MKX expression was significantly increased in PDGF-BB and mixed groups and COLI expression was significantly increased only in PDGF-BB group. In conclusion, our results showed that PDGF-BB and GDF-6 combination could induce tenogenic differentiation in eASCs. These in vitro findings could be useful for cell therapy in equine regenerative medicine.

Identifiants

DOI: 10.1007/s11033-020-05742-7 PMID: 32875433
pubmed: 32875433
doi: 10.1007/s11033-020-05742-7
pii: 10.1007/s11033-020-05742-7
doi:

Substances chimiques

Basic Helix-Loop-Helix Transcription Factors 0
Collagen Type I 0
Growth Differentiation Factor 6 0
Homeodomain Proteins 0
Becaplermin 1B56C968OA

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

6855-6862

Références

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Auteurs

Shabnam Javanshir (S)

Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

Fatemeh Younesi Soltani (F)

Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

Gholamreza Dowlati (G)

Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

Abbas Parham (A)

Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. parham@um.ac.ir.
Stem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Azadi Square, Mashhad, 9177948974, Iran. parham@um.ac.ir.
ORCID: 0000-0003-4664-2917

Hojjat Naderi-Meshkin (H)

Stem Cells and Regenerative Medicine Research Group, Iranian Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Induction of tenogenic differentiation of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription à motif basique hélice-boucle-hélice › Induction of tenogenic differentiation of...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Facteur de croissance dérivé des plaquettes › Protéines proto-oncogènes c-sis › Bécaplermine › Induction of tenogenic differentiation of...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Induction of tenogenic differentiation of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Protéines de la matrice extracellulaire › Collagène › Collagènes fibrillaires › Collagène de type I › Induction of tenogenic differentiation of...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes au cours du développement › Induction of tenogenic differentiation of...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Protéines de la superfamille du TGF bêta › Facteurs de croissance et de différenciation › Facteur-6 de croissance et de différenciation › Induction of tenogenic differentiation of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de liaison à l'ADN › Protéines à homéodomaine › Induction of tenogenic differentiation of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Perissodactyla › Equidae › Equus caballus › Induction of tenogenic differentiation of...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › Induction of tenogenic differentiation of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Induction of tenogenic differentiation of...
questionsmedicales.fr › Appareil locomoteur › Tendons › Induction of tenogenic differentiation of...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Ingénierie tissulaire › Induction of tenogenic differentiation of...