Diffusion Limited Cryopreservation of Tissue with Radiofrequency Heated Metal Forms.
2 mm thick tissue systems
porcine aortas
tissue cryopreservation
Journal
Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
11
05
2020
revised:
15
07
2020
pubmed:
3
9
2020
medline:
15
5
2021
entrez:
3
9
2020
Statut:
ppublish
Résumé
Cryopreserved tissues are increasingly needed in biomedical applications. However, successful cryopreservation is generally only reported for thin tissues (≤1 mm). This work presents several innovations to reduce cryoprotectant (CPA) toxicity and improve tissue cryopreservation, including 1) improved tissue warming rates through radiofrequency metal form and field optimization and 2) an experimentally verified predictive model to optimize CPA loading and rewarming to reduce toxicity. CPA loading is studied by microcomputed tomography (µCT) imaging, rewarming by thermal measurements, and modeling, and viability is measured after loading and/or cryopreservation by alamarBlue and histology. Loading conditions for three common CPA cocktails (6, 8.4, and 9.3 m) are designed, and then fast cooling and metal forms rewarming (up to 2000 °C min
Identifiants
pubmed: 32875732
doi: 10.1002/adhm.202000796
pmc: PMC7879698
mid: NIHMS1639381
doi:
Substances chimiques
Cryoprotective Agents
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000796Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK117425
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL135046
Pays : United States
Informations de copyright
© 2020 The Authors. Published by Wiley-VCH GmbH.
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