The Fragility of Cryopreserved Insulin-producing Cells Differentiated from Adipose-tissue-derived Stem Cells.
Adenosine Triphosphate
/ metabolism
Adipose Tissue
/ cytology
Caspase 3
/ metabolism
Cell Differentiation
/ drug effects
Cell Shape
/ drug effects
Cell Survival
/ drug effects
Cryopreservation
Dithizone
Glucose
/ pharmacology
Humans
Insulin
/ metabolism
Insulin Secretion
/ drug effects
Insulin-Secreting Cells
/ cytology
Staining and Labeling
Stem Cells
/ cytology
adipose-derived stem cell
cryopreservation
insulin-producing cell
regenerative medicine
Journal
Cell transplantation
ISSN: 1555-3892
Titre abrégé: Cell Transplant
Pays: United States
ID NLM: 9208854
Informations de publication
Date de publication:
Historique:
entrez:
4
9
2020
pubmed:
4
9
2020
medline:
25
6
2021
Statut:
ppublish
Résumé
The aim of our study is to determine whether insulin-producing cells (IPCs) differentiated from adipose-tissue-derived stem cells (ADSCs) can be cryopreserved. Human ADSCs were differentiated into IPCs using our two-step protocol encompassing a three-dimensional culture and xenoantigen-free method. Thereafter, IPCs were frozen using three different methods. First, IPCs were immediately frozen at -80°C (-80°C group). Second, IPCs were initially placed into a Bicell freezing container before freezing at -80°C (BICELL group). Third, a vitrification method for oocytes and embryos was used (CRYOTOP group). Cell counting kit-8 (CCK-8) assay showed that cell viability was decreased in all groups after cryopreservation (
Identifiants
pubmed: 32878465
doi: 10.1177/0963689720954798
pmc: PMC7784513
doi:
Substances chimiques
Insulin
0
Dithizone
60-10-6
Adenosine Triphosphate
8L70Q75FXE
Caspase 3
EC 3.4.22.-
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
963689720954798Références
Cryobiology. 1993 Aug;30(4):407-12
pubmed: 8403991
Curr Stem Cell Res Ther. 2019;14(1):52-56
pubmed: 30207243
Islets. 2013 Sep-Dec;5(5):188-95
pubmed: 24759005
Pancreas. 2008 May;36(4):417-23
pubmed: 18437089
Transplantation. 2001 Sep 27;72(6):1005-11
pubmed: 11579292
Islets. 2018 Jan 2;10(1):40-49
pubmed: 29315020
Clin Sci (Lond). 2006 Jun;110(6):611-25
pubmed: 16689680
J Artif Organs. 2014 Dec;17(4):337-43
pubmed: 24944122
J Artif Organs. 2012 Sep;15(3):283-9
pubmed: 22382647
Sci Rep. 2019 Jul 24;9(1):10759
pubmed: 31341242
Cryobiology. 1988 Feb;25(1):1-17
pubmed: 3280245
Diabetes. 1989 Jan;38 Suppl 1:176-8
pubmed: 2642844
Diabetologia. 1992 Jan;35(1):89-95
pubmed: 1541386
Diabetes Res Clin Pract. 2014 Feb;103(2):137-49
pubmed: 24630390
Sci Rep. 2019 Dec 10;9(1):18731
pubmed: 31822724
Transplantation. 2002 Dec 27;74(12):1687-91
pubmed: 12499881
Cell Transplant. 2008;17(1-2):61-7
pubmed: 18468236
Ann N Y Acad Sci. 1999 Jun 18;875:200-7
pubmed: 10415568
Pancreas. 2018 Apr;47(4):477-481
pubmed: 29517636
Transplantation. 1991 Jan;51(1):76-85
pubmed: 1987709
Cryobiology. 1987 Apr;24(2):91-102
pubmed: 3568745
Surgery. 2012 Mar;151(3):412-9
pubmed: 21982637
Transplantation. 1999 Sep 15;68(5):597-8
pubmed: 10507475
Transplantation. 1999 Sep 15;68(5):655-62
pubmed: 10507485
Pancreas. 2010 Oct;39(7):960-3
pubmed: 20467350
Transplantation. 2010 Jan 15;89(1):47-54
pubmed: 20061918
Diabetes. 2005 Jul;54(7):2060-9
pubmed: 15983207
J Surg Res. 2006 Jun 15;133(2):167-75
pubmed: 16360174
Cryobiology. 1977 Oct;14(5):549-58
pubmed: 908192
Transpl Int. 2016 Sep;29(9):1039-50
pubmed: 27306931
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Diabetologia. 1991 Jan;34(1):55-8
pubmed: 2055341