Targeted Metagenomics for Clinical Detection and Discovery of Bacterial Tick-Borne Pathogens.
Lyme disease
anaplasmosis
human granulocytic ehrlichiosis
metagenomics
tick-borne bacteria
vector-borne diseases
Journal
Journal of clinical microbiology
ISSN: 1098-660X
Titre abrégé: J Clin Microbiol
Pays: United States
ID NLM: 7505564
Informations de publication
Date de publication:
21 10 2020
21 10 2020
Historique:
received:
23
01
2020
accepted:
21
07
2020
pubmed:
4
9
2020
medline:
24
6
2021
entrez:
4
9
2020
Statut:
epublish
Résumé
Tick-borne diseases, due to a diversity of bacterial pathogens, represent a significant and increasing public health threat throughout the Northern Hemisphere. A high-throughput 16S V1-V2 rRNA gene-based metagenomics assay was developed and evaluated using >13,000 residual samples from patients suspected of having tick-borne illness and >1,000 controls. Taxonomic predictions for tick-borne bacteria were exceptionally accurate, as independently validated by secondary testing. Overall, 881 specimens were positive for bacterial tick-borne agents. Twelve tick-borne bacterial species were detected, including two novel pathogens, representing a 100% increase in the number of tick-borne bacteria identified compared to what was possible by initial PCR testing. In three blood specimens, two tick-borne bacteria were simultaneously detected. Seven bacteria, not known to be tick transmitted, were also confirmed to be unique to samples from persons suspected of having tick-borne illness. These results indicate that 16S V1-V2 metagenomics can greatly simplify diagnosis and accelerate the discovery of bacterial tick-borne pathogens.
Identifiants
pubmed: 32878950
pii: JCM.00147-20
doi: 10.1128/JCM.00147-20
pmc: PMC7587092
pii:
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 American Society for Microbiology.
Références
Emerg Infect Dis. 1998 Apr-Jun;4(2):179-86
pubmed: 9621188
Clin Infect Dis. 2018 Jun 1;66(12):1864-1871
pubmed: 29272385
Diagn Microbiol Infect Dis. 2012 Jul;73(3):243-5
pubmed: 22571973
J Clin Microbiol. 2002 Feb;40(2):690-3
pubmed: 11825999
Emerg Infect Dis. 2008 Mar;14(3):522-3
pubmed: 18325285
Clin Microbiol Rev. 2005 Jul;18(3):484-509
pubmed: 16020686
Lancet Infect Dis. 2016 May;16(5):556-564
pubmed: 26856777
Genome Biol. 2014 Mar 03;15(3):R46
pubmed: 24580807
N Engl J Med. 2018 Aug 23;379(8):701-703
pubmed: 30044925
Trop Med Infect Dis. 2018 Jan 29;3(1):
pubmed: 30274412
J Clin Microbiol. 2003 Dec;41(12):5492-9
pubmed: 14662930
Trends Parasitol. 2015 Nov;31(11):536-552
pubmed: 26458781
Can Vet J. 2013 Jun;54(6):565-72
pubmed: 24155447
N Engl J Med. 2011 Aug 4;365(5):422-9
pubmed: 21812671
Clin Microbiol Infect. 2011 Jan;17(1):69-79
pubmed: 20132258
Int J Syst Evol Microbiol. 2020 May;70(5):3577-3581
pubmed: 32320380
Trends Parasitol. 2018 Apr;34(4):295-309
pubmed: 29336985
Emerg Infect Dis. 2006 Nov;12(11):1799-801
pubmed: 17283646
Bioinformatics. 2011 Nov 1;27(21):2957-63
pubmed: 21903629
J Clin Microbiol. 2013 Jan;51(1):314-7
pubmed: 23135935
Clin Infect Dis. 2010 Feb 15;50(4):541-8
pubmed: 20073993
MMWR Morb Mortal Wkly Rep. 2018 May 04;67(17):496-501
pubmed: 29723166
Clin Infect Dis. 2014 Sep 1;59(5):676-81
pubmed: 24879782
Emerg Infect Dis. 2015 Sep;21(9):1625-31
pubmed: 26291194
Ticks Tick Borne Dis. 2018 Sep;9(6):1528-1531
pubmed: 30064965
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
Emerg Infect Dis. 2015 Oct;21(10):1794-9
pubmed: 26402378
Am J Clin Pathol. 2020 Jan 1;153(1):139-145
pubmed: 31584611
BMC Bioinformatics. 2014 Nov 19;15:366
pubmed: 25408143
J Clin Microbiol. 1997 Nov;35(11):2715-27
pubmed: 9350721
Ann Intern Med. 2013 Jul 2;159(1):21-7
pubmed: 23817701
BMC Biol. 2014 Nov 12;12:87
pubmed: 25387460
Ticks Tick Borne Dis. 2010 Dec;1(4):159-67
pubmed: 21359090