Establishment and Characterization of a Unilateral UV-Induced Photoreceptor Degeneration Model in the C57Bl/6J Mouse.
LIRD
UV-damage
animal model
light damage
retinal degeneration
Journal
Translational vision science & technology
ISSN: 2164-2591
Titre abrégé: Transl Vis Sci Technol
Pays: United States
ID NLM: 101595919
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
03
05
2019
revised:
01
11
2019
accepted:
21
11
2019
entrez:
4
9
2020
pubmed:
4
9
2020
medline:
4
9
2020
Statut:
epublish
Résumé
To investigate whether UV irradiation of the mouse eye can induce photoreceptor degeneration, producing a phenotype reminiscent of the rd10 mouse, left eyes of female C57Bl/6J mice were irradiated with a UV LED array (370 nm). A lens was placed between the cornea and LED, allowing illumination of about one-third of the retina. The short-term and long-term effects on the retina were evaluated. First, a dose escalation study, in which corneal dosages between 2.8 and 9.3 J/cm In treated eyes, spectral domain optical coherence tomography revealed a decrease in retinal thickness to 53%. Full-field electroretinography responses decreased significantly from day 5 on in treated eyes. Multielectrode array recordings revealed oscillatory potentials with a mean frequency of 5.2 UV irradiation proved to be efficient in inducing photoreceptor degeneration in the mouse retina, while leaving the other retinal layers largely intact. The irradiated area of treated eyes can be identified easily in spectral domain optical coherence tomography and in explanted retinae. This study provides information on anatomic and functional changes in UV-treated retina, enabling the use of this model for retinitis pigmentosa-like diseases in animals suited for experimental retinal surgery.
Identifiants
pubmed: 32879777
doi: 10.1167/tvst.9.9.21
pii: TVST-19-1603
pmc: PMC7443125
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
21Informations de copyright
Copyright 2020 The Authors.
Déclaration de conflit d'intérêts
Disclosure: A.-M. van der Meer, None; T. Berger, None; F. Müller, None; A.C. Foldenauer, None; S. Johnen, None; P. Walter, None
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