Phytoplankton composition in a eutrophic estuary: Comparison of multiple taxonomic approaches and influence of environmental factors.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
19
01
2020
revised:
24
08
2020
accepted:
27
08
2020
pubmed:
4
9
2020
medline:
10
4
2021
entrez:
4
9
2020
Statut:
ppublish
Résumé
To assess the comparability between taxonomic identification methods for phytoplankton, multiple approaches were used to characterize phytoplankton community composition within the Neuse River Estuary (NRE), North Carolina. Small subunit 18S rRNA gene sequencing and accessory pigment analysis displayed similar trends, indicating chlorophytes were the dominant microalgal group during most of the year, whereas results from microscopic cell counts, biovolume analysis and metatranscriptomics suggested diatom and dinoflagellate-dominated communities. Spatial environmental gradients drove variation in taxonomic composition due to preferences for specific environmental conditions among different microalgal groups. Cryptophytes were a greater proportion of the phytoplankton community within high nutrient, fresher environments whereas diatoms and dinoflagellates dominated higher salinity sections of the estuary. This study provides a detailed examination of phytoplankton communities associated with environmental gradients present in the NRE. The high level of taxonomic resolution offered by DNA sequencing (i.e., species to sub-species level) provides a better understanding of population dynamics at the base of estuarine food webs.
Identifiants
pubmed: 32881227
doi: 10.1111/1462-2920.15221
doi:
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4718-4731Subventions
Organisme : NC Sea Grant
ID : R/12-HCE-3-NA10OAR4170080
Organisme : NC Sea Grant
ID : R/14-HCE-2-NA14OAR4170073
Organisme : Department of Environment and Natural Resources, Division of Water Resources, the Lower Neuse Basin Association and Neuse River Compliance Association and National Fish and Wildlife Foundation
Informations de copyright
© 2020 Society for Applied Microbiology and John Wiley & Sons Ltd.
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