Tensile mechanical changes in the Achilles tendon due to Insertional Achilles tendinopathy.
Achilles tendon
Collagen
Glycosaminoglycans
Insertional achilles tendinopathy
Quasi-linear viscoelasticity (QLV)
Tensile
Journal
Journal of the mechanical behavior of biomedical materials
ISSN: 1878-0180
Titre abrégé: J Mech Behav Biomed Mater
Pays: Netherlands
ID NLM: 101322406
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
27
10
2019
revised:
05
03
2020
accepted:
07
08
2020
pubmed:
4
9
2020
medline:
15
5
2021
entrez:
4
9
2020
Statut:
ppublish
Résumé
Insertional Achilles tendinopathy (IAT) is a painful condition that is challenging to treat non-operatively. Although previous studies have characterized the gross histological features, in vivo strain patterns and transverse compressive mechanical properties of tissue affected by IAT, it is not known how IAT impacts the tensile mechanical properties of the Achilles tendon insertion along the axial/longitudinal direction (i.e., along the predominant direction of loading). To address this knowledge gap, the objectives of this study were to 1) apply ex vivo mechanical testing, nonlinear elastic analysis and quasilinear viscoelastic (QLV) analysis to compare the axial tensile mechanical properties of the Achilles tendon insertion in individuals with and without IAT; and 2) use biochemical analysis and second harmonic generation (SHG) imaging to assess structural and compositional changes induced by IAT in order to help explain IAT-associated tensile mechanical changes. Tissue from the Achilles tendon insertion was acquired from healthy donors and from patients undergoing debridement surgery for IAT. Tissue specimens were mechanically tested using a uniaxial tensile (stress relaxation) test applied in the axial direction. A subset of the donor specimens was used for SHG imaging and biochemical analysis. Linear and non-linear elastic analyses of the stress relaxation tests showed no significant tensile mechanical changes in IAT specimens compared to healthy controls. However, SHG analysis showed that fibrillar collagen was significantly more disorganized in IAT tissue as compared with healthy controls, and biochemical analysis showed that sulfated glycosaminoglycan (sGAG) content and water content were higher in IAT specimens. Collectively, these findings suggest that conservative interventions for IAT should target restoration of ultrastructural organization, reduced GAG content, and reduced resistance to transverse compressive strain.
Identifiants
pubmed: 32882677
pii: S1751-6161(20)30582-8
doi: 10.1016/j.jmbbm.2020.104031
pmc: PMC8056289
mid: NIHMS1626785
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
104031Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR070765
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
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