Intestinal stem cells and intestinal organoids.
Colorectal cancer genomics
Disease model
Genetic disorders
Genetic marker
Intestinal epithelium
Intestinal stem cell
Lineage tracing
Organoids
Journal
Journal of genetics and genomics = Yi chuan xue bao
ISSN: 1673-8527
Titre abrégé: J Genet Genomics
Pays: China
ID NLM: 101304616
Informations de publication
Date de publication:
20 06 2020
20 06 2020
Historique:
received:
24
03
2020
revised:
15
06
2020
accepted:
25
06
2020
pubmed:
5
9
2020
medline:
12
6
2021
entrez:
5
9
2020
Statut:
ppublish
Résumé
The intestinal epithelium is one of the most rapidly renewing tissues, which is fueled by stem cells at the base of the crypts. Strategies of genetic lineage tracing and organoids, which capture major features of original tissues, are powerful avenues for exploring the biology of intestinal stem cells in vivo and in vitro, respectively. The combination of intestinal organoid-culturing system and genetic modification approaches provides an attractive platform to uncover the mechanism of colorectal cancer and genetic disorders in the human minigut. Here, we will provide a comprehensive overview of studies on intestinal epithelium and intestinal stem cells. We will also review the applications of organoids and genetic markers in intestinal research studies. Furthermore, we will discuss the advantages and drawbacks of organoids as disease models compared with mice models and cell lines.
Identifiants
pubmed: 32883604
pii: S1673-8527(20)30111-9
doi: 10.1016/j.jgg.2020.06.005
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
289-299Informations de copyright
Copyright © 2020 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Ltd. All rights reserved.