Encapsulation of pectinase within polyacrylamide gel: characterization of its catalytic properties for continuous industrial uses.
Acrylamide
Biotechnology
Characterization
Encapsulation
N, N′-methylenebisacrylamide
Pectinase
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
06
05
2020
revised:
29
05
2020
accepted:
24
07
2020
entrez:
5
9
2020
pubmed:
5
9
2020
medline:
5
9
2020
Statut:
epublish
Résumé
Pectinase as a biocatalyst play a significant role in food and textile industries. In this study, the pectinase was immobilized by encapsulation within polyacrylamide gel to enhance its catalytic properties and ensure the reusability for continuous industrial processes. 9.5% acrylamide and 0.5% N, N'- methylenebisacrylamide concentration gave high percentage of pectinase immobilization yield within gel. The catalytic properties of immobilized pectinase was determined with comparison of soluble pectinase. The immobilization of pectinase within polyacrylamide gel didn't effect catalytic properties of pectinase and both the free and immobilized pectinase showed maximum pectinolytic activity at 45 °C and pH 10. The Michaelis-Menten kinetic behavior of pectinase was slightly changed after immobilization and immobilized pectinase showed somewhat higher K
Identifiants
pubmed: 32885065
doi: 10.1016/j.heliyon.2020.e04578
pii: S2405-8440(20)31422-5
pii: e04578
pmc: PMC7453123
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e04578Informations de copyright
© 2020 Published by Elsevier Ltd.
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