Denatonium benzoate bitter taste perception in chronic rhinosinusitis subgroups.
AERD
bitter taste receptors
bitterness
mucosal immunity
sensory perception
solitary chemosensory cells
type 2 inflammation
Journal
International forum of allergy & rhinology
ISSN: 2042-6984
Titre abrégé: Int Forum Allergy Rhinol
Pays: United States
ID NLM: 101550261
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
revised:
29
07
2020
received:
09
03
2020
accepted:
10
08
2020
pubmed:
5
9
2020
medline:
30
9
2021
entrez:
5
9
2020
Statut:
ppublish
Résumé
Chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) and CRS without nasal polyps (CRSsNP), and aspirin-exacerbated respiratory disease (AERD) have varying levels of inflammation and disease severity. Solitary chemosensory cells (SCCs) are enriched in nasal polyps, are the primary source of interleukin 25 (IL-25) in upper airways, leading to type 2 inflammation, and are activated by bitter-tasting denatonium benzoate (DB). Thus, we sought to evaluate DB taste perception at a range of concentrations in order to identify 1 that most differentiates CRS subgroups from controls. CRSsNP (n = 25), CRSwNP (n = 26), and AERD (n = 27) patients as well as controls (n = 25) tasted 6 DB concentrations in a fixed, random order, rating on a category scale of 0 (no intensity) to 12 (extremely intense). Sinonasal epithelial cultures were treated with and without denatonium and analyzed for IL-25 via flow cytometry. CRSsNP patients rated DB as significantly less intense than did controls at all concentrations: 5.62 × 10 Our findings link in vitro DB stimulation of sinonasal tissue with increased IL-25 and show differential DB taste perception in CRS subgroups relative to the control group, with CRSsNP being hyposensitive and AERD being hypersensitive. We propose a concentration of 3.16 × 10
Sections du résumé
BACKGROUND
Chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) and CRS without nasal polyps (CRSsNP), and aspirin-exacerbated respiratory disease (AERD) have varying levels of inflammation and disease severity. Solitary chemosensory cells (SCCs) are enriched in nasal polyps, are the primary source of interleukin 25 (IL-25) in upper airways, leading to type 2 inflammation, and are activated by bitter-tasting denatonium benzoate (DB). Thus, we sought to evaluate DB taste perception at a range of concentrations in order to identify 1 that most differentiates CRS subgroups from controls.
METHODS
CRSsNP (n = 25), CRSwNP (n = 26), and AERD (n = 27) patients as well as controls (n = 25) tasted 6 DB concentrations in a fixed, random order, rating on a category scale of 0 (no intensity) to 12 (extremely intense). Sinonasal epithelial cultures were treated with and without denatonium and analyzed for IL-25 via flow cytometry.
RESULTS
CRSsNP patients rated DB as significantly less intense than did controls at all concentrations: 5.62 × 10
CONCLUSION
Our findings link in vitro DB stimulation of sinonasal tissue with increased IL-25 and show differential DB taste perception in CRS subgroups relative to the control group, with CRSsNP being hyposensitive and AERD being hypersensitive. We propose a concentration of 3.16 × 10
Substances chimiques
Quaternary Ammonium Compounds
0
denatonium
4IK22DF4OU
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
967-975Subventions
Organisme : NCATS NIH HHS
ID : TL1TR001880
Pays : United States
Organisme : NIH HHS
ID : R01DC013588
Pays : United States
Organisme : NIH HHS
ID : S10 OD018125
Pays : United States
Informations de copyright
© 2020 ARS-AAOA, LLC.
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