Impact of the isoelectric point of model parvoviruses on viral retention in anion-exchange chromatography.
anion-exchange chromatography
isoelectric point
parvovirus
virus clearance
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
29
05
2020
revised:
14
08
2020
accepted:
31
08
2020
pubmed:
5
9
2020
medline:
26
11
2021
entrez:
5
9
2020
Statut:
ppublish
Résumé
Anion-exchange chromatography (AEX) is used in the downstream purification of monoclonal antibodies to remove impurities and potential viral contamination based on electrostatic interactions. Although the isoelectric point (pI) of viruses is considered a key factor predicting the virus adsorption to the resin, the precise molecular mechanisms involved remain unclear. To address this question, we compared structurally homologous parvoviruses that only differ in their surface charge distribution. A single charged amino acid substitution on the capsid surface of minute virus of mice (MVM) provoked an increased apparent pI (pI
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
116-129Informations de copyright
© 2020 Wiley Periodicals LLC.
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