Relative content detection of oligomannose modification of IgM heavy chain induced by TNP-antigen in an early vertebrate through nanoLC-MS/MS.

Antigen recognition IgM Oligomannose Oreochromis niloticus nanoLC-MS/MS

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
01 Nov 2020
Historique:
received: 20 04 2020
revised: 20 06 2020
accepted: 24 06 2020
entrez: 5 9 2020
pubmed: 6 9 2020
medline: 15 5 2021
Statut: ppublish

Résumé

N-glycan modification is reported to be important in regulating the structure and function of immunoglobulins in mammals. While, the study on teleost immunoglobulin glycosylation is still limitted. In this study, we constructed a TNP-antigen driven model, and detected the site-specific N-glycans of PBS-immunized and TNP-specific Oreochromis niloticus serum IgM through

Identifiants

pubmed: 32887075
pii: S0039-9140(20)30637-8
doi: 10.1016/j.talanta.2020.121346
pii:
doi:

Substances chimiques

Immunoglobulin M 0
Polysaccharides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

121346

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Auteurs

Xiaoxue Yin (X)

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, PR China.

Xiaoyu Li (X)

State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, 102206, PR China; College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100124, PR China.

Liangliang Mu (L)

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, PR China.

Kailiang Han (K)

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, PR China.

Hairong Wu (H)

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, PR China.

Liting Wu (L)

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, PR China.

Anli Wang (A)

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, PR China.

Wantao Ying (W)

State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, 102206, PR China. Electronic address: yingwantao@mail.ncpsb.org.

Jianmin Ye (J)

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, PR China. Electronic address: jmye@m.scnu.edu.cn.

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Classifications MeSH