Towards Mimicking the Fetal Liver Niche: The Influence of Elasticity and Oxygen Tension on Hematopoietic Stem/Progenitor Cells Cultured in 3D Fibrin Hydrogels.
Animals
Biomimetics
Cell Differentiation
Cell Proliferation
Cells, Cultured
Elasticity
Embryo, Mammalian
/ cytology
Fetus
/ cytology
Fibrin
/ chemistry
Hematopoietic Stem Cells
/ cytology
Hydrogels
/ chemistry
Liver
/ embryology
Mice
Mice, Inbred C57BL
Oxygen
/ metabolism
Stem Cell Niche
/ physiology
3D cell encapsulation
elastic modulus
fetal liver niche
fibrin hydrogel
hematopoietic stem cells
oxygen tension
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
02 Sep 2020
02 Sep 2020
Historique:
received:
29
07
2020
revised:
24
08
2020
accepted:
28
08
2020
entrez:
5
9
2020
pubmed:
6
9
2020
medline:
26
3
2021
Statut:
epublish
Résumé
Hematopoietic stem/progenitor cells (HSPCs) are responsible for the generation of blood cells throughout life. It is believed that, in addition to soluble cytokines and niche cells, biophysical cues like elasticity and oxygen tension are responsible for the orchestration of stem cell fate. Although several studies have examined the effects of bone marrow (BM) niche elasticity on HSPC behavior, no study has yet investigated the effects of the elasticity of other niche sites like the fetal liver (FL), where HSPCs expand more extensively. In this study, we evaluated the effect of matrix stiffness values similar to those of the FL on BM-derived HSPC expansion. We first characterized the elastic modulus of murine FL tissue at embryonic day E14.5. Fibrin hydrogels with similar stiffness values as the FL (soft hydrogels) were compared with stiffer fibrin hydrogels (hard hydrogels) and with suspension culture. We evaluated the expansion of total nucleated cells (TNCs), Lin
Identifiants
pubmed: 32887387
pii: ijms21176367
doi: 10.3390/ijms21176367
pmc: PMC7504340
pii:
doi:
Substances chimiques
Hydrogels
0
Fibrin
9001-31-4
Oxygen
S88TT14065
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Onderzoeksraad, KU Leuven
ID : IDO/13/016
Organisme : Fonds Wetenschappelijk Onderzoek
ID : G.0947.17N
Organisme : Fonds Wetenschappelijk Onderzoek
ID : 11C3821N
Organisme : Fonds Wetenschappelijk Onderzoek
ID : G.0E01.17N
Organisme : Consejo Nacional de Ciencia y Tecnología
ID : 326227 / 440074
Organisme : Fonds Wetenschappelijk Onderzoek
ID : G.0929.15
Organisme : Herculesstichting
ID : AKUL/15/37_GOH1816N
Organisme : Herculesstichting
ID : HER/09/021
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