Plasmodium falciparum gametocyte-induced volatiles enhance attraction of Anopheles mosquitoes in the field.
(E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate
Anopheles
Attraction
HMBPP
Mosquitoes
Journal
Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802
Informations de publication
Date de publication:
04 Sep 2020
04 Sep 2020
Historique:
received:
14
01
2020
accepted:
18
08
2020
entrez:
5
9
2020
pubmed:
6
9
2020
medline:
16
4
2021
Statut:
epublish
Résumé
Plasmodium parasites manipulate the interaction between their mosquito and human hosts. Patients infected with gametocytes attract anopheline mosquitoes differentially compared to healthy individuals, an effect associated with an increased release of attractive volatile cues. This odour-driven manipulation is partly mediated by the gametocyte-specific metabolite, (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), which induces increased release of select aldehydes and terpenes from red blood cells and results in the enhanced attraction of host-seeking mosquitoes, which are vectors of malaria. This study investigates the effect of the HMBPP-induced volatiles on the attraction of wild Anopheles mosquitoes to humans under field conditions. The efficacy of the HMBPP-induced odour blend to attract Anopheles was evaluated in a 4 × 6 Latin rectangular study design indoors using baited Suna traps. Furthermore, to assess the efficacy of the HMBPP-induced odour blend in (1) augmenting the attractiveness of human odour, and (2) attracting Anopheles mosquitoes in the absence of human odour, a two-choice assay using host decoy traps (HDTs) was used and evaluated using binomial generalized regression. Traps baited with the HMBPP-induced odour blend attracted and caught both Anopheles arabiensis and Anopheles pharoensis females in a dose-dependent manner in the presence of background human odour, up to 2.5 times that of an unbaited trap. Given a choice between human odour and human odour laden with the HMBPP-induced odour blend, mosquitoes differentially preferred traps augmented with the HMBPP-induced odour blend, which caught twice as many female An. arabiensis. Traps baited with the HMBPP-induced odour blend but lacking the background of human odour were not effective in attracting and catching mosquitoes. The findings of the present study revealed that the HMBPP-induced odour blend, when augmented with human body odour, is attractive to anopheline mosquitoes and could be used as a complementary vector control tool along with existing strategies.
Sections du résumé
BACKGROUND
BACKGROUND
Plasmodium parasites manipulate the interaction between their mosquito and human hosts. Patients infected with gametocytes attract anopheline mosquitoes differentially compared to healthy individuals, an effect associated with an increased release of attractive volatile cues. This odour-driven manipulation is partly mediated by the gametocyte-specific metabolite, (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), which induces increased release of select aldehydes and terpenes from red blood cells and results in the enhanced attraction of host-seeking mosquitoes, which are vectors of malaria. This study investigates the effect of the HMBPP-induced volatiles on the attraction of wild Anopheles mosquitoes to humans under field conditions.
METHODS
METHODS
The efficacy of the HMBPP-induced odour blend to attract Anopheles was evaluated in a 4 × 6 Latin rectangular study design indoors using baited Suna traps. Furthermore, to assess the efficacy of the HMBPP-induced odour blend in (1) augmenting the attractiveness of human odour, and (2) attracting Anopheles mosquitoes in the absence of human odour, a two-choice assay using host decoy traps (HDTs) was used and evaluated using binomial generalized regression.
RESULTS
RESULTS
Traps baited with the HMBPP-induced odour blend attracted and caught both Anopheles arabiensis and Anopheles pharoensis females in a dose-dependent manner in the presence of background human odour, up to 2.5 times that of an unbaited trap. Given a choice between human odour and human odour laden with the HMBPP-induced odour blend, mosquitoes differentially preferred traps augmented with the HMBPP-induced odour blend, which caught twice as many female An. arabiensis. Traps baited with the HMBPP-induced odour blend but lacking the background of human odour were not effective in attracting and catching mosquitoes.
CONCLUSION
CONCLUSIONS
The findings of the present study revealed that the HMBPP-induced odour blend, when augmented with human body odour, is attractive to anopheline mosquitoes and could be used as a complementary vector control tool along with existing strategies.
Identifiants
pubmed: 32887614
doi: 10.1186/s12936-020-03378-3
pii: 10.1186/s12936-020-03378-3
pmc: PMC7487932
doi:
Substances chimiques
4-hydroxy-3-methylbut-2-enyl pyrophosphate
0
Diphosphates
0
Volatile Organic Compounds
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
327Références
Parasit Vectors. 2014 May 29;7:251
pubmed: 24885914
PLoS One. 2013 May 15;8(5):e63602
pubmed: 23691073
Malar J. 2010 Jul 10;9:198
pubmed: 20618990
Malar J. 2016 Aug 11;15(1):404
pubmed: 27515306
J Infect Dis. 2018 Apr 23;217(10):1553-1560
pubmed: 29415208
Malar J. 2018 Oct 5;17(1):351
pubmed: 30290799
Sci Rep. 2017 Dec 11;7(1):17283
pubmed: 29229938
Parasite Epidemiol Control. 2017 May 02;2(2):61-69
pubmed: 29774282
Proc Natl Acad Sci U S A. 2018 May 1;115(18):E4209-E4218
pubmed: 29666273
J Infect Dis. 2017 Aug 1;216(3):291-295
pubmed: 28859429
Science. 2017 Mar 10;355(6329):1076-1080
pubmed: 28183997
PLoS Biol. 2005 Sep;3(9):e298
pubmed: 16076240
Sci Rep. 2016 Nov 30;6:37930
pubmed: 27901056
Sci Rep. 2017 Aug 24;7(1):9283
pubmed: 28839251
Sci Rep. 2019 Jul 4;9(1):9699
pubmed: 31273284
Lancet. 2016 Sep 17;388(10050):1193-201
pubmed: 27520594
Malar J. 2013 Jun 27;12:218
pubmed: 23805822