3D Ultrastructural Study of Synapses in the Human Entorhinal Cortex.
FIB-SEM
cerebral cortex
electron microscopy
neuropil
synaptology
Journal
Cerebral cortex (New York, N.Y. : 1991)
ISSN: 1460-2199
Titre abrégé: Cereb Cortex
Pays: United States
ID NLM: 9110718
Informations de publication
Date de publication:
01 01 2021
01 01 2021
Historique:
received:
02
06
2020
revised:
24
07
2020
accepted:
28
07
2020
pubmed:
6
9
2020
medline:
11
1
2022
entrez:
5
9
2020
Statut:
ppublish
Résumé
The entorhinal cortex (EC) is a brain region that has been shown to be essential for memory functions and spatial navigation. However, detailed three-dimensional (3D) synaptic morphology analysis and identification of postsynaptic targets at the ultrastructural level have not been performed before in the human EC. In the present study, we used Focused Ion Beam/Scanning Electron Microscopy to perform a 3D analysis of the synapses in the neuropil of medial EC in layers II and III from human brain autopsies. Specifically, we studied synaptic structural parameters of 3561 synapses, which were fully reconstructed in 3D. We analyzed the synaptic density, 3D spatial distribution, and type (excitatory and inhibitory), as well as the shape and size of each synaptic junction. Moreover, the postsynaptic targets of synapses could be clearly determined. The present work constitutes a detailed description of the synaptic organization of the human EC, which is a necessary step to better understand the functional organization of this region in both health and disease.
Identifiants
pubmed: 32887978
pii: 5901490
doi: 10.1093/cercor/bhaa233
pmc: PMC7727377
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
410-425Informations de copyright
© The Author(s) 2020. Published by Oxford University Press.
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