Cell cycle dynamics of lamina-associated DNA.
DamID
cell cycle
genome organization
nuclear lamina
telomeres
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
05 11 2020
05 11 2020
Historique:
received:
14
04
2020
revised:
07
08
2020
accepted:
11
08
2020
pubmed:
8
9
2020
medline:
28
4
2021
entrez:
7
9
2020
Statut:
ppublish
Résumé
In mammalian interphase nuclei, more than one thousand large genomic regions are positioned at the nuclear lamina (NL). These lamina-associated domains (LADs) are involved in gene regulation and may provide a backbone for the folding of interphase chromosomes. Little is known about the dynamics of LADs during interphase, in particular at the onset of G1 phase and during DNA replication. We developed an antibody-based variant of the DamID technology (named pA-DamID) that allows us to map and visualize genome-NL interactions with high temporal resolution. Application of pA-DamID combined with synchronization and cell sorting experiments reveals that LAD-NL contacts are generally rapidly established early in G1 phase. However, LADs on the distal ~25 Mb of most chromosomes tend to contact the NL first and then gradually detach, while centromere-proximal LADs accumulate gradually at the NL. Furthermore, our data indicate that S-phase chromatin shows transiently increased lamin interactions. These findings highlight a dynamic choreography of LAD-NL contacts during interphase progression and illustrate the usefulness of pA-DamID to study the dynamics of genome compartmentalization.
Identifiants
pubmed: 32893442
doi: 10.15252/embr.202050636
pmc: PMC7645246
doi:
Substances chimiques
Chromatin
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e50636Subventions
Organisme : NIDDK NIH HHS
ID : U54 DK107965
Pays : United States
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
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