Comparative performance of two commercial sample-to-result systems for hepatitis C virus quantitation and genotyping.
Hepatitis C virus
diagnostic test evaluation
genotype 6
genotyping
real-time RT-PCR
Journal
Expert review of molecular diagnostics
ISSN: 1744-8352
Titre abrégé: Expert Rev Mol Diagn
Pays: England
ID NLM: 101120777
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
pubmed:
8
9
2020
medline:
16
10
2021
entrez:
7
9
2020
Statut:
ppublish
Résumé
Accurate assays for hepatitis C virus (HCV) quantitation and genotyping are important for the management of HCV infection. In this study, we evaluated the performance of cobas HCV and cobas HCV GT assays (Roche) for HCV quantitation and genotyping on the cobas 4800 System. We compared the performance of the cobas HCV assays with another commercial system (Abbott The limit-of-detection of the cobas HCV assay in our center was higher (15 IU/mL) than the manufacturer claim (9.2 IU/mL). The assay showed high analytical specificity, accuracy, precision, and linearity. Performance was congruent with the RealTi Our results confirm that the cobas 4800 System is a reliable platform for HCV quantitation and genotyping. The cobas HCV GT assay is a good choice for genotype 1b/6 endemic areas in east Asia, clearly outperforming the RealTi
Identifiants
pubmed: 32893699
doi: 10.1080/14737159.2020.1820327
doi:
Substances chimiques
RNA, Viral
0
Reagent Kits, Diagnostic
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM