Midazolam oxidation in cattle liver microsomes: The role of cytochrome P450 3A.
CYP3A
cattle
drug metabolism
midazolam
Journal
Journal of veterinary pharmacology and therapeutics
ISSN: 1365-2885
Titre abrégé: J Vet Pharmacol Ther
Pays: England
ID NLM: 7910920
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
13
05
2020
revised:
24
07
2020
accepted:
13
08
2020
pubmed:
8
9
2020
medline:
19
5
2021
entrez:
7
9
2020
Statut:
ppublish
Résumé
In humans, the cytochrome P450 3A (CYP3A) subfamily is involved in midazolam (MDZ) biotransformation into 1'- and 4-hydroxy metabolites, and the former serves as a probe for CYP3A catalytic activity. In veterinary species is still crucial to identify enzyme- and species-specific CYP substrates; thus, the aim of this study was to characterize MDZ oxidation in cattle liver. A HPLC-UV method was used to measure 1'- and 4-hydroxy MDZ (1'- and 4-OHMDZ, respectively) formation in cattle liver microsomes and assess the role of CYP3A by an immunoinhibition study. Moreover, MDZ hydroxylation was evaluated in 300 cattle liver samples and results were correlated with testosterone hydroxylation. Formation of both metabolites conformed to a single-enzyme Michaelis-Menten kinetics. Values of V
Substances chimiques
Adjuvants, Anesthesia
0
Cytochrome P-450 CYP3A
EC 1.14.14.1
Midazolam
R60L0SM5BC
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
608-613Subventions
Organisme : Italian Ministry of Education, University and Research (MIUR)
ID : 2009ZE5HJP
Informations de copyright
© 2020 John Wiley & Sons Ltd.
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