Adipose Tissue From Type 1 Diabetes Mellitus Patients Can Be Used to Generate Insulin-Producing Cells.
Adipose Tissue
/ cytology
Animals
Cells, Cultured
Diabetes Mellitus, Experimental
/ metabolism
Diabetes Mellitus, Type 1
/ metabolism
Glucose
/ pharmacology
Humans
Insulin
/ metabolism
Insulin Secretion
/ drug effects
Insulin-Secreting Cells
/ cytology
Mice, Nude
Microscopy, Electron, Transmission
Stem Cell Transplantation
/ methods
Stem Cells
/ cytology
Journal
Pancreas
ISSN: 1536-4828
Titre abrégé: Pancreas
Pays: United States
ID NLM: 8608542
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
pubmed:
9
9
2020
medline:
10
8
2021
entrez:
8
9
2020
Statut:
ppublish
Résumé
We aimed to determine whether responsive insulin-producing cells (IPCs) could be generated from adipose-derived stem cells (ADSCs) isolated from patients with type 1 diabetes mellitus (T1DM). We isolated ADSCs from adipose tissue of 4 patients (one patient with T1DM and 3 nondiabetic patients), who underwent surgery and differentiated them into IPCs with using a 2-step xeno-antigen free, 3-dimensional culture method. Characteristics of isolated ADSCs, in vitro cell quality, programmed cell death ligand-1 (PDL-1) expression, and transplantation into streptozotocin induced diabetic nude mice were investigated. Adipose-derived stem cells from T1DM patients and commercially obtained ADSCs showed the same surface markers; CD31CD34CD45CD90CD105CD146. Moreover, the generated IPCs at day 21 demonstrated appropriate autonomous insulin secretion (stimulation index, 3.5; standard deviation, 0.8). Nonfasting blood glucose concentrations of IPC-transplanted mice were normal at 30 days. The normalized rate of IPC-transplanted mice was significantly higher than that of the sham-operated group (P < 0.05). Insulin-producing cells generated from T1DM adipose tissue expressed high levels of PDL-1. Insulin-producing cells obtained from adipose tissue of T1DM patients are capable of secreting insulin long-term and achieve normoglycemia after transplantation. Expression of PDL-1 suggests the potential for immune circumvention.
Identifiants
pubmed: 32898009
doi: 10.1097/MPA.0000000000001663
pii: 00006676-202010000-00015
doi:
Substances chimiques
Insulin
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1225-1231Références
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