Prevascularized Scaffolds Bearing Human Dental Pulp Stem Cells for Treating Complete Spinal Cord Injury.


Journal

Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613

Informations de publication

Date de publication:
10 2020
Historique:
received: 09 06 2020
revised: 08 08 2020
pubmed: 10 9 2020
medline: 15 5 2021
entrez: 9 9 2020
Statut: ppublish

Résumé

The regeneration of injured spinal cord is hampered by the lack of vascular supply and neurotrophic support. Transplanting tissue-engineered constructs with developed vascular networks and neurotrophic factors, and further understanding the pattern of vessel growth in the remodeled spinal cord tissue are greatly desired. To this end, highly vascularized scaffolds embedded with human dental pulp stem cells (DPSCs) are fabricated, which possess paracrine-mediated angiogenic and neuroregenerative potentials. The potent pro-angiogenic effect of the prevascularized scaffolds is first demonstrated in a rat femoral bundle model, showing robust vessel growth and blood perfusion induced within these scaffolds postimplantation, as evidenced by laser speckle contrast imaging and 3D microCT dual imaging modalities. More importantly, in a rat complete spinal cord transection model, the implantation of these scaffolds to the injured spinal cords can also promote revascularization, as well as axon regeneration, myelin deposition, and sensory recovery. Furthermore, 3D microCT imaging and novel morphometric analysis on the remodeled spinal cord tissue demonstrate substantial regenerated vessels, more significantly in the sensory tract regions, which correlates with behavioral recovery following prevascularization treatment. Taken together, prevascularized DPSC-embedded constructs bear angiogenic and neurotrophic potentials, capable of augmenting and modulating SCI repair.

Identifiants

pubmed: 32902147
doi: 10.1002/adhm.202000974
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2000974

Informations de copyright

© 2020 Wiley-VCH GmbH.

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Auteurs

Shaowei Guo (S)

Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.
The First Affiliated Hospital, Shantou University Medical College, Shantou, 515000, China.

Idan Redenski (I)

Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.

Shira Landau (S)

Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.

Ariel Szklanny (A)

Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.

Uri Merdler (U)

Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.

Shulamit Levenberg (S)

Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.

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