Oligonucleotide Probe for Transcriptome
Animals
Biotin
/ analogs & derivatives
Brain
/ cytology
Carbocyanines
/ chemistry
Fluorescent Dyes
/ chemistry
Gene Expression Profiling
/ methods
Light
Mice
Microscopy, Confocal
/ methods
Microscopy, Fluorescence
/ methods
Neurons
/ metabolism
Nitrobenzenes
/ chemistry
RNA Probes
/ chemistry
RNA, Messenger
/ analysis
Single-Cell Analysis
/ methods
Transcriptome
Journal
ACS chemical biology
ISSN: 1554-8937
Titre abrégé: ACS Chem Biol
Pays: United States
ID NLM: 101282906
Informations de publication
Date de publication:
16 10 2020
16 10 2020
Historique:
pubmed:
10
9
2020
medline:
2
6
2021
entrez:
9
9
2020
Statut:
ppublish
Résumé
Messenger RNA (mRNA) isolated from single cells can generate powerful biological insights, including the discovery of new cell types with unique functions as well as markers potentially predicting a cell's response to various therapeutic agents. We previously introduced an oligonucleotide-based technique for site-selective, photoinduced biotinylation and capture of mRNA within a living cell called transcriptome
Identifiants
pubmed: 32902259
doi: 10.1021/acschembio.0c00499
pmc: PMC8312762
mid: NIHMS1726454
doi:
Substances chimiques
Carbocyanines
0
Fluorescent Dyes
0
Nitrobenzenes
0
RNA Probes
0
RNA, Messenger
0
cyanine dye 3
0
Biotin
6SO6U10H04
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2714-2721Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM083030
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM131907
Pays : United States
Références
Nucleic Acids Res. 2015 Oct 30;43(19):e126
pubmed: 26101260
Cell. 2015 May 21;161(5):1202-1214
pubmed: 26000488
Nat Commun. 2020 Jun 16;11(1):3055
pubmed: 32546686
Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):11046-51
pubmed: 27625426
Neurosci Bull. 2017 Dec;33(6):763-765
pubmed: 28780643
Nat Protoc. 2017 Jan;12(1):44-73
pubmed: 27929523
Anal Chem. 2019 Sep 3;91(17):11374-11379
pubmed: 31402646
Biotechnol Prog. 2008 Jul-Aug;24(4):957-63
pubmed: 19194904
Sci Rep. 2019 Dec 9;9(1):18618
pubmed: 31819112
BMC Biol. 2017 May 19;15(1):44
pubmed: 28526029
J Am Chem Soc. 2020 Jul 1;142(26):11343-11356
pubmed: 32573219
Proc Natl Acad Sci U S A. 2019 Sep 24;116(39):19490-19499
pubmed: 31501331
Science. 2006 Sep 29;313(5795):1929-35
pubmed: 17008526
Lancet. 2003 Aug 2;362(9381):362-9
pubmed: 12907009
Science. 2017 Jun 9;356(6342):
pubmed: 28473638
Org Biomol Chem. 2017 Dec 6;15(47):10001-10009
pubmed: 29052679
Development. 2017 Oct 1;144(19):3625-3632
pubmed: 28851704
Nat Methods. 2017 Oct;14(10):955-958
pubmed: 28846088
J Am Chem Soc. 2015 Dec 2;137(47):14838-41
pubmed: 26587747
Nat Protoc. 2015 Mar;10(3):442-58
pubmed: 25675209
Int J Pharm. 2013 Jan 30;441(1-2):242-7
pubmed: 23200958
J Am Chem Soc. 2012 Jan 25;134(3):1376-91
pubmed: 22229439
Genome Biol. 2019 Aug 6;20(1):155
pubmed: 31387612
Proc Natl Acad Sci U S A. 2013 May 7;110(19):7625-30
pubmed: 23613589
Nat Methods. 2014 Feb;11(2):190-6
pubmed: 24412976
Proc Natl Acad Sci U S A. 2015 Aug 4;112(31):9591-5
pubmed: 26195734
Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):4340-4344
pubmed: 29632214
J Am Soc Nephrol. 2019 Jan;30(1):23-32
pubmed: 30510133
Genome Med. 2016 Dec 23;8(1):133
pubmed: 28007021
Nature. 2019 Apr;568(7751):235-239
pubmed: 30911168
Mol Cell. 2019 Mar 21;73(6):1292-1305.e8
pubmed: 30765193
Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10787-92
pubmed: 11553813
Genome Biol. 2016 Apr 28;17:77
pubmed: 27121950
Analyst. 2018 Jul 9;143(14):3292-3301
pubmed: 29708557
Nucleic Acids Res. 2019 Jun 20;47(11):e66
pubmed: 30923815
Nat Biotechnol. 2014 Dec;32(12):1202-12
pubmed: 24880487
J Am Chem Soc. 2013 Sep 4;135(35):12952-5
pubmed: 23931073