Designing Safer CRISPR/Cas9 Therapeutics for HIV: Defining Factors That Regulate and Technologies Used to Detect Off-Target Editing.
BLISS
CIRCLE-Seq
CRISPR/Cas9
DISCOVER-Seq
GUIDE-Seq
human immunodeficiency virus
off-target
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2020
2020
Historique:
received:
17
01
2020
accepted:
16
07
2020
entrez:
9
9
2020
pubmed:
10
9
2020
medline:
10
9
2020
Statut:
epublish
Résumé
Human immunodeficiency virus type-1 (HIV-1) infection has resulted in the death of upward of 39 million people since being discovered in the early 1980s. A cure strategy for HIV-1 has eluded scientists, but gene editing technologies such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) offer a new approach to developing a cure for HIV infection. While the CRISPR/Cas9 system has been used successfully in a number of different types of studies, there remains a concern for off-target effects. This review details the different aspects of the Cas9 system and how they play a role in off-target events. In addition, this review describes the current technologies available for detecting off-target cleavage events and their advantages and disadvantages. While some studies have utilized whole genome sequencing (WGS), this method sacrifices depth of coverage for interrogating the whole genome. A number of different approaches have now been developed to take advantage of next generation sequencing (NGS) without sacrificing depth of coverage. This review highlights four widely used methods for detecting off-target events: (1) genome-wide unbiased identification of double-stranded break events enabled by sequencing (GUIDE-Seq), (2) discovery of
Identifiants
pubmed: 32903440
doi: 10.3389/fmicb.2020.01872
pmc: PMC7434968
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
1872Subventions
Organisme : NIMH NIH HHS
ID : P30 MH092177
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH110360
Pays : United States
Organisme : NIMH NIH HHS
ID : T32 MH079785
Pays : United States
Informations de copyright
Copyright © 2020 Sullivan, Allen, Atkins, Chung, Dampier, Nonnemacher and Wigdahl.
Références
Science. 2019 Apr 19;364(6437):286-289
pubmed: 31000663
Nature. 2016 Jan 28;529(7587):490-5
pubmed: 26735016
Sci Rep. 2016 Mar 04;6:22555
pubmed: 26939770
Nat Biotechnol. 2015 Feb;33(2):187-197
pubmed: 25513782
Nat Rev Mol Cell Biol. 2017 Aug;18(8):495-506
pubmed: 28512351
Nat Biotechnol. 2014 Jul;32(7):670-6
pubmed: 24752079
Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3707-11
pubmed: 7731969
PLoS Pathog. 2019 Feb 27;15(2):e1007619
pubmed: 30811499
Nat Biotechnol. 2013 Sep;31(9):822-6
pubmed: 23792628
Nature. 2011 Mar 31;471(7340):602-7
pubmed: 21455174
Nat Rev Microbiol. 2011 Jun;9(6):467-77
pubmed: 21552286
Science. 2020 Feb 28;367(6481):
pubmed: 32029687
Nature. 2018 Sep;561(7723):416-419
pubmed: 30209390
BMC Biol. 2018 Jul 11;16(1):75
pubmed: 29996827
Viruses. 2019 Mar 13;11(3):
pubmed: 30871200
MOJ Immunol. 2014 Oct 17;1(4):
pubmed: 25893217
Nat Biotechnol. 2013 Sep;31(9):839-43
pubmed: 23934178
Cell. 2015 Aug 27;162(5):1113-26
pubmed: 26317473
Nature. 2015 Apr 9;520(7546):186-91
pubmed: 25830891
Nat Biotechnol. 2013 Mar;31(3):233-9
pubmed: 23360965
Nucleic Acids Res. 2018 Jan 9;46(1):350-361
pubmed: 29145633
Nat Biotechnol. 2016 Feb;34(2):184-191
pubmed: 26780180
Proc Natl Acad Sci U S A. 2014 Aug 5;111(31):11461-6
pubmed: 25049410
Proc Natl Acad Sci U S A. 2012 Sep 25;109(39):E2579-86
pubmed: 22949671
Nat Biotechnol. 2015 Dec;33(12):1293-1298
pubmed: 26524662
Nat Med. 2018 Aug;24(8):1216-1224
pubmed: 30082871
Nucleic Acids Res. 2015 Oct 15;43(18):e118
pubmed: 26032770
Mol Ther Nucleic Acids. 2018 Sep 7;12:275-282
pubmed: 30195766
Gene Ther. 2016 Aug;23(8-9):690-5
pubmed: 27194423
Sci Rep. 2019 Mar 8;9(1):3928
pubmed: 30850620
Nat Biotechnol. 2014 Jul;32(7):677-83
pubmed: 24837660
Nat Biotechnol. 2013 Sep;31(9):827-32
pubmed: 23873081
Nat Commun. 2019 Jul 2;10(1):2753
pubmed: 31266936
Annu Rev Biophys. 2017 May 22;46:505-529
pubmed: 28375731
Nature. 2015 Jul 23;523(7561):481-5
pubmed: 26098369
Cell Rep. 2018 Feb 6;22(6):1413-1423
pubmed: 29425498
Nature. 1992 Nov 19;360(6401):264-5
pubmed: 1436108
Mol Ther. 2020 Jan 8;28(1):19-28
pubmed: 31672284
Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):9798-803
pubmed: 24912165
Nat Methods. 2017 Jun;14(6):607-614
pubmed: 28459458
Sci Rep. 2019 Nov 19;9(1):17088
pubmed: 31745112
BMC Genomics. 2018 Mar 21;19(1):212
pubmed: 29562890
ACS Chem Biol. 2018 Feb 16;13(2):397-405
pubmed: 29083855
Nat Biotechnol. 2015 Feb;33(2):179-86
pubmed: 25503383
Nucleic Acids Res. 2018 Jan 25;46(2):792-803
pubmed: 29216382
Nat Biotechnol. 2015 Feb;33(2):175-8
pubmed: 25599175
Nat Biotechnol. 2014 Mar;32(3):279-284
pubmed: 24463574
Science. 2016 Jan 1;351(6268):84-8
pubmed: 26628643
Intensive Care Med. 1999 Feb;25(2):198-206
pubmed: 10193548
CRISPR J. 2018 Aug;1:294-302
pubmed: 31021222
PLoS One. 2016 May 19;11(5):e0155382
pubmed: 27195985
Sci Rep. 2016 Aug 16;6:31527
pubmed: 27528385
Science. 2012 Aug 17;337(6096):816-21
pubmed: 22745249
Nat Commun. 2017 May 12;8:15058
pubmed: 28497783
Mol Ther. 2016 Mar;24(3):508-21
pubmed: 26775808
AIDS Res Hum Retroviruses. 2018 Nov;34(11):950-960
pubmed: 29968495
Nat Biotechnol. 2013 Sep;31(9):833-8
pubmed: 23907171