A stability-indicating UPLC method for the determination of curcumin diethyl disuccinate, an ester prodrug of curcumin, in raw materials.
Analytical chemistry
Chromatography
Curcumin diethyl disuccinate
Optical spectroscopy
Pharmaceutical chemistry
Pharmaceutical science
Prodrug
Stability-indicating method
UPLC
Validation
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
20
05
2020
revised:
05
07
2020
accepted:
22
07
2020
entrez:
9
9
2020
pubmed:
10
9
2020
medline:
10
9
2020
Statut:
epublish
Résumé
A stability-indicating reversed-phase ultra-performance liquid chromatographic (UPLC) method for quantitative analysis of curcumin diethyl disuccinate (CDD) in raw materials was developed for applications in product development and quality control. Chromatographic separation was performed using the Waters ACQUITY UPLC® H-Class system consisting of an ACQUITY UPLC® BEH C18 (1.7 μm, 2.1 × 50 mm) column and a photodiode array detector set at a wavelength of 400 nm. The mobile phase consisting of 2%v/v acetic acid in water and acetonitrile was delivered at a flow rate of 0.3 mL/min under gradient elution program. The method was validated according to the ICH Q2(R1) guideline for the validation of analytical procedures. The method was found to be linear over the concentration range of 8-12 μg/mL with the coefficient of determination >0.995. The accuracy of the method established as %recovery ranged from 98.3 - 100.8%. The precision of the method expressed as %CV was found to be <1%. The coelution of degradation products from six stress test conditions was not observed. The method was robust under the variation of chromatographic parameters. The method was successfully applied in the determination of CDD content in raw materials.
Identifiants
pubmed: 32904269
doi: 10.1016/j.heliyon.2020.e04561
pii: S2405-8440(20)31405-5
pii: e04561
pmc: PMC7452553
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e04561Informations de copyright
© 2020 The Authors.
Références
Molecules. 2011 Feb 22;16(2):1888-900
pubmed: 21343891
J Pharm Biomed Anal. 1997 Aug;15(12):1867-76
pubmed: 9278892
Pharmazie. 2016 Dec 1;71(12):691-700
pubmed: 29441997
J Biol Chem. 2015 Feb 20;290(8):4817-28
pubmed: 25564617
Sci Rep. 2019 Aug 12;9(1):11718
pubmed: 31406217
J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Oct 15;1033-1034:301-310
pubmed: 27595650
Pharmaceutics. 2019 Aug 01;11(8):
pubmed: 31374932
Eur J Drug Metab Pharmacokinet. 2016 Dec;41(6):777-785
pubmed: 26563392