Remarkable migration propensity of dental pulp stem cells towards neurodegenerative milieu: An in vitro analysis.
Dental pulp stem cells
Hippocampus
Mesenchymal stem cells
Migration
Neurodegeneration
Stromal cell derived factor-alpha
Journal
Neurotoxicology
ISSN: 1872-9711
Titre abrégé: Neurotoxicology
Pays: Netherlands
ID NLM: 7905589
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
29
02
2020
revised:
25
07
2020
accepted:
30
08
2020
pubmed:
10
9
2020
medline:
13
10
2021
entrez:
9
9
2020
Statut:
ppublish
Résumé
Stem cell therapy provides a ray of hope for treating neurodegenerative diseases (ND). Bone marrow mesenchymal stem cells (BM-MSC) were extensively investigated for their role in neuroregeneration. However, drawbacks like painful bone marrow extraction, less proliferation and poor CNS engraftment following systemic injections of BM-MSC prompt us to search for alternate/appropriate source of MSC for treating ND. In this context, dental pulp stem cells (DPSC) could be an alternative to BM-MSC as it possess both mesenchymal and neural characteristic features due to its origin from ectoderm, ease of isolation, higher proliferation index and better neuroprotection. A study on the migration potential of DPSC compared to BM-MSC in a neurodegenerative condition is warranted. Given the neural crest origin, we hypothesize that DPSC possess better migration towards neurodegenerative milieu as compared to BM-MSC. In this prospect, we investigated the migration potential of DPSC in an in vitro neurodegenerative condition. Towards this, transwell, Matrigel and chorioallantoic membrane (CAM) migration assays were carried-out by seeding hippocampal neurons in the lower chamber and treated with 300 μM kainic acid (KA) for 6 h to induce neurodegeneration. Subsequently, the upper chamber of transwell was loaded with DPSC/BM-MSC and their migration potential was assessed following 24 h of incubation. Our results revealed that the migration potential of DPSC/BM-MSC was comparable in non-degenerative condition. However, following injury the migration potential of DPSC towards the degenerating site was significantly higher as compared to BM-MSC. Furthermore, upon exposure of naïve DPSC/BM-MSCs to culture medium derived from neurodegenerative milieu resulted in significant upregulation of homing factors like SDF-1alpha, CXCR-4, VCAM-1, VLA-4, CD44, MMP-2 suggesting that the superior migration potential of DPSC might be due to prompt expression of homing factors in DPSC compared to BM-MSCs.
Identifiants
pubmed: 32905802
pii: S0161-813X(20)30130-3
doi: 10.1016/j.neuro.2020.08.006
pii:
doi:
Substances chimiques
Culture Media, Conditioned
0
Kainic Acid
SIV03811UC
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
89-100Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.