Cholestasis Differentially Affects Liver Connexins.
Animals
Bile Acids and Salts
/ metabolism
Bile Ducts
/ metabolism
Cells, Cultured
Cholestasis
/ metabolism
Connexin 26
/ metabolism
Connexin 43
/ metabolism
Connexins
/ metabolism
Hepatocytes
/ metabolism
Humans
Liver
/ pathology
Male
Mice
Mice, Inbred C57BL
RNA, Messenger
/ metabolism
Gap Junction beta-1 Protein
cholestasis
connexin
liver
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
07 Sep 2020
07 Sep 2020
Historique:
received:
03
07
2020
revised:
26
08
2020
accepted:
05
09
2020
entrez:
10
9
2020
pubmed:
11
9
2020
medline:
23
2
2021
Statut:
epublish
Résumé
Connexins are goal keepers of tissue homeostasis, including in the liver. As a result, they are frequently involved in disease. The current study was set up to investigate the effects of cholestatic disease on the production of connexin26, connexin32 and connexin43 in the liver. For this purpose, bile duct ligation, a well-known trigger of cholestatic liver injury, was applied to mice. In parallel, human hepatoma HepaRG cell cultures were exposed to cholestatic drugs and bile acids. Samples from both the in vivo and in vitro settings were subsequently subjected to assessment of mRNA and protein quantities as well as to in situ immunostaining. While the outcome of cholestasis on connexin26 and connexin43 varied among experimental settings, a more generalized repressing effect was seen for connexin32. This has also been observed in many other liver pathologies and could suggest a role for connexin32 as a robust biomarker of liver disease and toxicity.
Identifiants
pubmed: 32906817
pii: ijms21186534
doi: 10.3390/ijms21186534
pmc: PMC7116118
mid: EMS94842
pii:
doi:
Substances chimiques
Bile Acids and Salts
0
Connexin 43
0
Connexins
0
RNA, Messenger
0
Connexin 26
127120-53-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Research Council
ID : 861913
Pays : International
Organisme : University Hospital of the Vrije Universiteit Brussel-Belgium
ID : "Willy Gepts Fonds" UZ-VUB
Organisme : Fonds Wetenschappelijk Onderzoek
ID : G009514N, G010214N and G012318N
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : FAPESP SPEC grant 2013/50420-6 and FAPESP-FWO grant 18/10953-9
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