Cholesterol transport between red blood cells and lipoproteins contributes to cholesterol metabolism in blood.
adenosine 5′-triphosphate binding cassette transporter A1
apolipoprotein A-I
cholesterol flux
cholesterol/metabolism
erythrocyte
high density lipoprotein
lipidomics
low density lipoprotein
Journal
Journal of lipid research
ISSN: 1539-7262
Titre abrégé: J Lipid Res
Pays: United States
ID NLM: 0376606
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
pubmed:
11
9
2020
medline:
26
10
2021
entrez:
10
9
2020
Statut:
ppublish
Résumé
Lipoproteins play a key role in transport of cholesterol to and from tissues. Recent studies have also demonstrated that red blood cells (RBCs), which carry large quantities of free cholesterol in their membrane, play an important role in reverse cholesterol transport. However, the exact role of RBCs in systemic cholesterol metabolism is poorly understood. RBCs were incubated with autologous plasma or isolated lipoproteins resulting in a significant net amount of cholesterol moved from RBCs to HDL, while cholesterol from LDL moved in the opposite direction. Furthermore, the bi-directional cholesterol transport between RBCs and plasma lipoproteins was saturable and temperature-, energy-, and time-dependent, consistent with an active process. We did not find LDLR, ABCG1, or scavenger receptor class B type 1 in RBCs but found a substantial amount of ABCA1 mRNA and protein. However, specific cholesterol efflux from RBCs to isolated apoA-I was negligible, and ABCA1 silencing with siRNA or inhibition with vanadate and Probucol did not inhibit the efflux to apoA-I, HDL, or plasma. Cholesterol efflux from and cholesterol uptake by RBCs from
Identifiants
pubmed: 32907987
pii: S0022-2275(20)60020-7
doi: 10.1194/jlr.RA120000635
pmc: PMC7707172
pii:
doi:
Substances chimiques
Lipoproteins
0
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1577-1588Déclaration de conflit d'intérêts
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
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