Osteoconductive and electroactive carbon nanofibers/hydroxyapatite nanocomposite tailored for bone tissue engineering: in vitro and in vivo studies.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 09 2020
09 09 2020
Historique:
received:
09
07
2020
accepted:
13
08
2020
entrez:
10
9
2020
pubmed:
11
9
2020
medline:
16
3
2021
Statut:
epublish
Résumé
In this study, we aimed to fabricate osteoconductive electrospun carbon nanofibers (CNFs) decorated with hydroxyapatite (HA) crystal to be used as the bone tissue engineering scaffold in the animal model. CNFs were derived from electrospun polyacrylonitrile (PAN) nanofibers via heat treatment and the carbonized nanofibers were mineralized by a biomimetic approach. The growth of HA crystals was confirmed using XRD, FTIR, and EDAX analysis techniques. The mineralization process turned the hydrophobic CNFs (WCA: 133.5° ± 0.6°) to hydrophilic CNFs/HA nanocomposite (WCA 15.3° ± 1°). The in vitro assessments revealed that the fabricated 24M-CNFs nanocomposite was biocompatible. The osteoconductive characteristics of CNFs/HA nanocomposite promoted in vivo bone formation in the rat's femur defect site, significantly, observed by computed tomography (CT) scan images and histological evaluation. Moreover, the histomorphometric analysis showed the highest new bone formation (61.3 ± 4.2%) in the M-CNFs treated group, which was significantly higher than the negative control group (the defect without treatment) (< 0.05). To sum up, the results implied that the fabricated CNFs/HA nanocomposite could be considered as the promising bone healing material.
Identifiants
pubmed: 32908157
doi: 10.1038/s41598-020-71455-3
pii: 10.1038/s41598-020-71455-3
pmc: PMC7481198
doi:
Substances chimiques
Carbon
7440-44-0
Durapatite
91D9GV0Z28
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14853Références
Aragón, J., Salerno, S., De Bartolo, L., Irusta, S. & Mendoza, G. Polymeric electrospun scaffolds for bone morphogenetic protein 2 delivery in bone tissue engineering. J. Colloid Interface Sci. 531, 126–137 (2018).
pubmed: 30029031
Ye, K. et al. Three-dimensional electrospun nanofibrous scaffolds displaying bone morphogenetic protein-2-derived peptides for the promotion of osteogenic differentiation of stem cells and bone regeneration. J. Colloid Interface Sci. 534, 625–636 (2019).
pubmed: 30265990
Samadian, H., Maleki, H., Allahyari, Z. & Jaymand, M. Natural polymers-based light-induced hydrogels: promising biomaterials for biomedical applications. Coord. Chem. Rev. 420, 213432 (2020).
Torres-Giner, S., Pérez-Masiá, R. & Lagaron, J. M. A review on electrospun polymer nanostructures as advanced bioactive platforms. Polym. Eng. Sci. 56, 500–527 (2016).
Rau, J. V., Antoniac, I., Cama, G., Komlev, V. S. & Ravaglioli, A. Bioactive materials for bone tissue engineering. BioMed Res. Int. https://doi.org/10.1155/2016/3741428 (2016).
doi: 10.1155/2016/3741428
pubmed: 28078286
pmcid: 5204102
Baino, F., Caddeo, S., Novajra, G. & Vitale-Brovarone, C. Using porous bioceramic scaffolds to model healthy and osteoporotic bone. J. Eur. Ceram. Soc. 36, 2175–2182 (2016).
Balasubramanian, P., Buettner, T., Pacheco, V. M. & Boccaccini, A. R. Boron-containing bioactive glasses in bone and soft tissue engineering. J. Eur. Ceram. Soc. 38, 855–869 (2018).
Yi, H., Rehman, F. U., Zhao, C., Liu, B. & He, N. Recent advances in nano scaffolds for bone repair. Bone Res. 4, 16050 (2016).
pubmed: 28018707
pmcid: 5153570
Santos, D. et al. Multifunctional PLLA-ceramic fiber membranes for bone regeneration applications. J. Colloid Interface Sci. 504, 101–110 (2017).
pubmed: 28531647
Boanini, E. et al. Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses. J. Colloid Interface Sci. 448, 1–7 (2015).
pubmed: 25706198
Pasteris, J. D., Wopenka, B. & Valsami-Jones, E. Bone and tooth mineralization: why apatite?. Elements 4, 97–104 (2008).
Sadat-Shojai, M., Khorasani, M.-T. & Jamshidi, A. A new strategy for fabrication of bone scaffolds using electrospun nano-HAp/PHB fibers and protein hydrogels. Chem. Eng. J. 289, 38–47 (2016).
Teotia, A. K. et al. Nano-hydroxyapatite bone substitute functionalized with bone active molecules for enhanced cranial bone regeneration. ACS Appl. Mater. Interfaces 9, 6816–6828 (2017).
pubmed: 28171719
Samadian, H. et al. Electro-conductive carbon nanofibers as the promising interfacial biomaterials for bone tissue engineering. J. Mol. Liq. 298, 112021 (2020).
Mata, D. et al. Smart electroconductive bioactive ceramics to promote in situ electrostimulation of bone. J. Mater. Chem. B 3, 1831–1845 (2015).
pubmed: 32262256
Chen, J., Yu, M., Guo, B., Ma, P. X. & Yin, Z. Conductive nanofibrous composite scaffolds based on in-situ formed polyaniline nanoparticle and polylactide for bone regeneration. J. Colloid Interface Sci. 514, 517–527 (2018).
pubmed: 29289734
Tian, J., Shi, Y., Fan, W. & Liu, T. Ditungsten carbide nanoparticles embedded in electrospun carbon nanofiber membranes as flexible and high-performance supercapacitor electrodes. Composites Commun. 12, 21–25 (2019).
Cai, J., Khatri, S. & Naraghi, M. Electrospun carbon nanofiber with controllable waviness as stretchable conductor. (2018).
Zhao, D. et al. Electrospun carbon nanofiber modified electrodes for stripping voltammetry. Anal. Chem. 87, 9315–9321 (2015).
pubmed: 26255824
Mirzaei, E. et al. The differentiation of human endometrial stem cells into neuron-like cells on electrospun PAN-derived carbon nanofibers with random and aligned topographies. Mol. Neurobiol. 53, 4798–4808 (2016).
pubmed: 26334615
Rajzer, I., Kwiatkowski, R., Piekarczyk, W., Biniaś, W. & Janicki, J. Carbon nanofibers produced from modified electrospun PAN/hydroxyapatite precursors as scaffolds for bone tissue engineering. Mater. Sci. Eng. C 32, 2562–2569 (2012).
Charbonnier, B., Laurent, C. & Marchat, D. Porous hydroxyapatite bioceramics produced by impregnation of 3D-printed wax mold: slurry feature optimization. J. Eur. Ceram. Soc. 36, 4269–4279 (2016).
Esslinger, S. & Gadow, R. Additive manufacturing of bioceramic scaffolds by combination of FDM and slip casting. J. Eur. Ceram. Soc. 40, 3707–3713 (2019).
Hajiali, F., Tajbakhsh, S. & Shojaei, A. Fabrication and properties of polycaprolactone composites containing calcium phosphate-based ceramics and bioactive glasses in bone tissue engineering: a review. Polym. Rev. 58, 164–207 (2018).
Pasuri, J. et al. Osteoclasts in the interface with electrospun hydroxyapatite. Colloids Surf. B 135, 774–783 (2015).
Hezma, A., El-Rafei, A., El-Bahy, G. & Abdelrazzak, A. B. Electrospun hydroxyapatite containing polyvinyl alcohol nanofibers doped with nanogold for bone tissue engineering. Interceram Int. Ceram. Rev. 66, 96–100 (2017).
Wu, M., Wang, Q., Liu, X. & Liu, H. Biomimetic synthesis and characterization of carbon nanofiber/hydroxyapatite composite scaffolds. Carbon 51, 335–345 (2013).
Zhao, B., Hu, H., Mandal, S. K. & Haddon, R. C. A bone mimic based on the self-assembly of hydroxyapatite on chemically functionalized single-walled carbon nanotubes. Chem. Mater. 17, 3235–3241 (2005).
Koutsopoulos, S. Synthesis and characterization of hydroxyapatite crystals: a review study on the analytical methods. J. Biomed. Mater. Res. 62, 600–612 (2002).
pubmed: 12221709
Kikuchi, M., Itoh, S., Ichinose, S., Shinomiya, K. & Tanaka, J. Self-organization mechanism in a bone-like hydroxyapatite/collagen nanocomposite synthesized in vitro and its biological reaction in vivo. Biomaterials 22, 1705–1711 (2001).
pubmed: 11396873
Rusu, V. M. et al. Size-controlled hydroxyapatite nanoparticles as self-organized organic–inorganic composite materials. Biomaterials 26, 5414–5426 (2005).
pubmed: 15814140
Samadian, H., Mobasheri, H., Hasanpour, S. & Faridi-Majid, R. Needleless electrospinning system, an efficient platform to fabricate carbon nanofibers. J. Nano Res. 50, 78–89 (2017).
Ślósarczyk, A., Paszkiewicz, Z. & Paluszkiewicz, C. FTIR and XRD evaluation of carbonated hydroxyapatite powders synthesized by wet methods. J. Mol. Struct. 744, 657–661 (2005).
Rhee, S. H. & Tanaka, J. Hydroxyapatite coating on a collagen membrane by a biomimetic method. J. Am. Ceram. Soc. 81, 3029–3031 (1998).
Granja, P., Ribeiro, C., De Jeso, B., Baquey, C. & Barbosa, M. Mineralization of regenerated cellulose hydrogels. J. Mater. Sci. Mater. Med. 12, 785–791 (2001).
pubmed: 15348225
Li, P. et al. The role of hydrated silica, titania, and alumina in inducing apatite on implants. J. Biomed. Mater. Res. 28, 7–15 (1994).
pubmed: 8126031
Ramier, J. et al. Biocomposite scaffolds based on electrospun poly (3-hydroxybutyrate) nanofibers and electrosprayed hydroxyapatite nanoparticles for bone tissue engineering applications. Mater. Sci. Eng. C 38, 161–169 (2014).
Liu, W. et al. Enhancing the stiffness of electrospun nanofiber scaffolds with a controlled surface coating and mineralization. Langmuir 27, 9088–9093 (2011).
pubmed: 21710996
pmcid: 3144316
Xie, J., Zhong, S., Ma, B., Shuler, F. D. & Lim, C. T. Controlled biomineralization of electrospun poly (ε-caprolactone) fibers to enhance their mechanical properties. Acta Biomater. 9, 5698–5707 (2013).
pubmed: 23131385
Wang, S., Yuan, T., Zhou, J. & Wang, Q.-Y. In 3rd International Conference on Electric and Electronics (Atlantis Press, 2013).
Hienz, S. A., Paliwal, S. & Ivanovski, S. Mechanisms of bone resorption in periodontitis. J. Immunol. Res. https://doi.org/10.1155/2015/615486 (2015).
doi: 10.1155/2015/615486
pubmed: 26065002
pmcid: 4433701
Miyamoto, T. & Suda, T. Differentiation and function of osteoclasts. Keio J. Med. 52, 1–7 (2003).
pubmed: 12713016
Henriksen, K., Bollerslev, J., Everts, V. & Karsdal, M. Osteoclast activity and subtypes as a function of physiology and pathology—implications for future treatments of osteoporosis. Endocr. Rev. 32, 31–63 (2011).
pubmed: 20851921
Hayakawa, S. et al. Heterogeneous structure and in vitro degradation behavior of wet-chemically derived nanocrystalline silicon-containing hydroxyapatite particles. Acta Biomater. 9, 4856–4867 (2013).
pubmed: 22922250
Matsumoto, T. et al. Hydroxyapatite particles as a controlled release carrier of protein. Biomaterials 25, 3807–3812 (2004).
pubmed: 15020156
Sheikh, Z. et al. Mechanisms of in vivo degradation and resorption of calcium phosphate based biomaterials. Materials 8, 7913–7925 (2015).
pubmed: 28793687
pmcid: 5458904
Dias, A., Gibson, I. R., Santos, J. & Lopes, M. Physicochemical degradation studies of calcium phosphate glass ceramic in the CaO–P2O5–MgO–TiO
pubmed: 17150421
Chen, X., Deng, C., Tang, S. & Zhang, M. Mitochondria-dependent apoptosis induced by nanoscale hydroxyapatite in human gastric cancer SGC-7901 cells. Biol. Pharm. Bull. 30, 128–132 (2007).
pubmed: 17202672
Meena, R., Kesari, K. K., Rani, M. & Paulraj, R. Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human breast cancer cells (MCF-7). J. Nanopart. Res. 14, 712 (2012).
Samadian, H. et al. Effective parameters on conductivity of mineralized carbon nanofibers: an investigation using artificial neural networks. RSC Adv. 6, 111908–111918 (2016).