Blutaparon portulacoides ethanolic extract reduced IL-1β and inflammatory parameters induced by the Mycobacterium complex and carrageenan in mice.
Amaranthaceae
/ chemistry
Animals
Anti-Inflammatory Agents
/ administration & dosage
Antitubercular Agents
/ administration & dosage
Carrageenan
Disease Models, Animal
Dose-Response Relationship, Drug
Edema
/ drug therapy
Female
Inflammation
/ drug therapy
Interleukin-1beta
/ metabolism
Male
Mice
Mice, Inbred C57BL
Microbial Sensitivity Tests
Mycobacterium tuberculosis
/ drug effects
Plant Extracts
/ administration & dosage
Pleurisy
/ drug therapy
Rats
Rats, Wistar
Amaranthaceae
Blutaparon portulacoides
Inflammation
Mycobacterium tuberculosis
Journal
Inflammopharmacology
ISSN: 1568-5608
Titre abrégé: Inflammopharmacology
Pays: Switzerland
ID NLM: 9112626
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
08
06
2020
accepted:
28
08
2020
pubmed:
11
9
2020
medline:
6
11
2021
entrez:
10
9
2020
Statut:
ppublish
Résumé
Information on the health benefits of ethanolic extracts obtained from Blutaparon portulacoides stem (EEBP) hasn´t been consistently described in the literature until the present moment. This study investigated the antimycobacterial, anti-inflammatory and toxicological effects of EEBP in models of inflammation/infection, as well as its chemical composition. Chemical analysis of EEBP by electrospray ionization-mass spectrometry/HPLC-MS/MS identified 3,5,3'-Trihydroxy-4'-methoxy-6,7-methylenedioxy-flavone, gomphrenol, ferulic, vanillic, and caffeic acids. The minimum inhibitory concentration of EEBP and isoniazid in the presence of Mycobacterium tuberculosis was 123.4 and 0.030 µg/ml, respectively. EEBP oral administration (p.o.) (300-1000 mg/kg) or dexamethasone subcutaneous injection (s.c.) (1 mg/kg) significantly inhibited leukocytes and proteins resulting from carrageenan-induced pleurisy in Swiss mice. In the BCG-induced pleurisy model, the oral treatments performed once a day for 7 days, with EEBP (30 and 100 mg/kg) and isoniazid (25 mg/kg), inhibited the increase in plasmatic IL-1β levels and in pleural exudate from C57BL-6 mice, and reduced M. tuberculosis growth in organs (colony forming units assays). EEBP (30-300 mg/kg, p.o.) and dexamethasone (1 mg/s.c.) significantly prevented carrageenan-induced oedema and mechanical hyperalgesia in Swiss mice. The treatments (once a day for 22 days) with EEBP (30 mg/kg, p.o.) and dexamethasone (1 mg/s.c.) substantially inhibited oedema and mechanical- and cold-hyperalgesia at 11, 16 and 22 days after the administration of Freund's Complete Adjuvant in C57bL6 mice. No evidence of physio-pathologic was observed in Wistar rats acutely treated with EEBP (2000 mg/kg, p.o.). This study confirms the anti-inflammatory and antibiotic properties of EEBP, opening possibilities for the development of safe new drugs with dual anti-inflammatory/antimycobacterial activities which could be favorable from a pharmacoeconomic perspective.
Identifiants
pubmed: 32910315
doi: 10.1007/s10787-020-00752-0
pii: 10.1007/s10787-020-00752-0
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Antitubercular Agents
0
Interleukin-1beta
0
Plant Extracts
0
Carrageenan
9000-07-1
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
439-450Subventions
Organisme : Fapesp
ID : Proc. 15/03726-8 and 06/06079-4
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